Please use this identifier to cite or link to this item: http://hdl.handle.net/10524/19034

Production Of Heterozygous And Homozygous Clones Of Common Carp (Cyprinus Carpio L.): Evidence From DNA Fingerprinting And Mixed Leukocyte Reaction

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Title: Production Of Heterozygous And Homozygous Clones Of Common Carp (Cyprinus Carpio L.): Evidence From DNA Fingerprinting And Mixed Leukocyte Reaction
Authors: Ben-Dom, Naomi
Cherfas. Nina B.
Keywords: common carp, Cyprinus carpio, DNA fingerprinting, heterozygosity, homozygosity, mixed leukocyte reaction
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.
Issue Date: 2001
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Ben-Dom, N., & Cherfas, N.B. (2001). Production Of Heterozygous And Homozygous Clones Of Common Carp (Cyprinus Carpio L.): Evidence From DNA Fingerprinting And Mixed Leukocyte Reaction. The Israeli Journal of Aquaculture - Bamidgeh, 53(3 - 4), 89-100.
Series/Report no.: The Israeli Journal of Aquaculture - Bamidgeh
Abstract: The production of two heterozygous and one homozygous clones of the Israeli Dor-70 line of com- mon carp (Cyprinus carpio L.) was tested using DNA fingerprinting (DFP) and mixed leukocyte reaction (MLR) analysis. The clones were obtained in large-scale production from mitotic gyno- genetic females and males (sex-inversed). MLR analysis was used to examine major histocom- patibility complex (MHC) antigens in individuals. DFP provided evidence concerning identity in the whole genome. Both methods showed that individuals within a progeny were identical, confirming genetic uniformity within each clone and showing that no paternal DNA was transmitted during mitotic and meiotic gynogenesis. The results indicated that uniformity of DFP in progeny of the homozygous clone is obtained only when the mother is fully homozygous, and in the heterozygous clone only if both parents are fully homozygous. The results of both methods provide evidence, though indirect, that the mitotic gynogenetic parents of these fish (mother of the homozygous clone, mother and father of the heterozygous clones) were fully homozygous.
Pages/Duration: 12 pages
URI/DOI: http://hdl.handle.net/10524/19034
ISSN: 0792-156X
Appears in Collections:IJA Volume 53, Issue 3-4, 2001



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