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Title: Milt Production Of Sea Bass Lates Calcarifer Bloch Administered An Analogue Of Luteinizing Hormone-Releasing Hormone And 17α-Methyltestosterone
Authors: Hilomen-Garcia, G.V.
Baldevarona, R.B.
Lacanilao, F.
Keywords: hormone, LHRHa, methyltestosterone, milt, sea bass
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Issue Date: 2002
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Hilomen-Garcia, G.V., Baldevarona, R.B., & Lacanilao, F. (2002). Milt Production Of Sea Bass Lates Calcarifer Bloch Administered An Analogue Of Luteinizing Hormone-Releasing Hormone And 17α-Methyltestosterone. The Israeli Journal of Aquaculture - Bamidgeh, 54(4), 173-182.
Series/Report no.: The Israeli Journal of Aquaculture - Bamidgeh
Abstract: The milt production responses of sexually mature sea bass Lates calcarifer to (D-Ala6, Pro9-N- ethylamide) luteinizing hormone-releasing hormone (LHRHa) and 17α-methyltestosterone injec- tions were examined.
At 24 h after injection of a low dose of LHRHa (20 μg/kg BW), the sperm count decreased significantly compared to saline-treated fish, but it returned to pre-treatment levels 48 h after injection, suggesting a possible hydration of the milt. Other milt parameters (milt volume, sper- matocrit, sperm production) in LHRHa-treated fish did not vary from their controls at 24 or 48 h after injection but the overall pattern suggested a reduction in milt viscosity. Total expressible milt and spermatozoa collected over the 48-h experiment was approximately three-fold higher in LHRHa-injected fish than in saline-injected fish, indicating a stimulation of spermatozoa produc- tion, not merely milt dilution due to hydration.
In a second experiment, sperm count and spermatocrit were significantly lower than those of saline-injected fish at 17 and 48 h after a single injection of a high dose of LHRHa (80 μg/kg BW). A methyltestosterone injection combined with the LHRHa injection also resulted in a significant- ly lower sperm count, but the spermatocrit remained comparable to the control group, suggest- ing a suppression of the LHRHa-induced milt hydration response.
Results demonstrate that LHRHa stimulates milt hydration and spermatozoa production in milting sea bass and that a simultaneous methyltestosterone injection partially suppresses this response.
Pages/Duration: 10 pages
ISSN: 0792-156X
Appears in Collections:IJA Volume 54, Issue 4, 2002

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