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Title: Production Of Androgenetic Nile Tilapia, Oreochromis Niloticus L.: Optimization Of Heat Shock Duration And Application Time To Induce Diploidy 
Author: Karayücel, Sedat; Karayücel, Ismihan; Penman, David; McAndrew, Brendan
Date: 2002
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Karayücel, S., Karayücel, I., Penman, D., & McAndrew, B. (2002). Production Of Androgenetic Nile Tilapia, Oreochromis Niloticus L.: Optimization Of Heat Shock Duration And Application Time To Induce Diploidy. The Israeli Journal of Aquaculture - Bamidgeh, 54(4), 145-156.
Abstract: Diploid androgenetic Nile tilapia, Oreochromis niloticus, were produced using 5 min UV irradia- tion at 150 μW/cm and heat shock at 42.5oC for 3:30 min applied 25 min after fertilization. The survival rates to morula, pigmentation, hatching and yolk sac resorption stages were 42.84±4.73%, 10.69±2.24%, 2.03±0.60% and 0.07±0.07% (relative to controls), respectively. A significant female, but not male, effect on the survival was confirmed. Multilocus DNA finger- printing produced by hypervariable 33.15 DNA probe verified the all-paternal inheritance in the androgens. The sex ratio of the androgenetic tilapia was not significantly different from the expected ratio of 1:0 (male:female), indicating that O. niloticus has a monofactorial sex-deter- mining mechanism with female homogamety and male heterogamety. This study confirmed that the YY genotype is viable and fertile in O. niloticus.
Series/Report No.: The Israeli Journal of Aquaculture - Bamidgeh
Pages/Duration: 12 pages
ISSN: 0792-156X
URI: http://hdl.handle.net/10524/19061
Keywords: androgenesis, diploidy, tilapia
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.

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