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Production Of Androgenetic Nile Tilapia, Oreochromis Niloticus L.: Optimization Of Heat Shock Duration And Application Time To Induce Diploidy
|Title:||Production Of Androgenetic Nile Tilapia, Oreochromis Niloticus L.: Optimization Of Heat Shock Duration And Application Time To Induce Diploidy|
|Keywords:||androgenesis, diploidy, tilapia|
|LC Subject Headings:||Fish culture--Israel--Periodicals.|
|Publisher:||Israeli Journal of Aquaculture - BAMIGDEH|
|Citation:||Karayücel, S., Karayücel, I., Penman, D., & McAndrew, B. (2002). Production Of Androgenetic Nile Tilapia, Oreochromis Niloticus L.: Optimization Of Heat Shock Duration And Application Time To Induce Diploidy. The Israeli Journal of Aquaculture - Bamidgeh, 54(4), 145-156.|
|Series/Report no.:||The Israeli Journal of Aquaculture - Bamidgeh|
|Abstract:||Diploid androgenetic Nile tilapia, Oreochromis niloticus, were produced using 5 min UV irradia- tion at 150 μW/cm and heat shock at 42.5oC for 3:30 min applied 25 min after fertilization. The survival rates to morula, pigmentation, hatching and yolk sac resorption stages were 42.84±4.73%, 10.69±2.24%, 2.03±0.60% and 0.07±0.07% (relative to controls), respectively. A significant female, but not male, effect on the survival was confirmed. Multilocus DNA finger- printing produced by hypervariable 33.15 DNA probe verified the all-paternal inheritance in the androgens. The sex ratio of the androgenetic tilapia was not significantly different from the expected ratio of 1:0 (male:female), indicating that O. niloticus has a monofactorial sex-deter- mining mechanism with female homogamety and male heterogamety. This study confirmed that the YY genotype is viable and fertile in O. niloticus.|
|Appears in Collections:||IJA Volume 54, Issue 4, 2002|
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