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dc.contributor.author Bozkurt, Yusuf en_US
dc.contributor.author Akcay, Ergun en_US
dc.contributor.author Tekin, Necmettin en_US
dc.contributor.author Secer, Selcuk en_US
dc.date.accessioned 2012-01-19T20:14:57Z en_US
dc.date.available 2012-01-19T20:14:57Z en_US
dc.date.issued 2005 en_US
dc.identifier.citation Bozkurt, Y., Akcay, E., Tekin, N., & Secer, S. (2005). Effect of freezing techniques, extenders and cryoprotectants on the fertilization rate of frozen rainbow trout (Oncorhynchus mykiss) sperm. The Israeli Journal of Aquaculture - Bamidgeh, 57(2), 125-130. en_US
dc.identifier.issn 0792-156X en_US
dc.identifier.uri http://hdl.handle.net/10524/19138 en_US
dc.description.abstract Rainbow trout (Oncorhynchus mykiss) sperm was frozen in liquid nitrogen in straws or as pellets on dry ice and stored in liquid nitrogen until artificial insemination. Sperm was diluted with one of three extenders containing 15% DMSO or 15% DMA as a cryoprotectant at a ratio of one part sperm to two parts extender. The straws were thawed in a water bath while the frozen pellets were thawed in their own extenders at 30oC for 30 s. For both freezing methods, the best fertil- ization results were obtained with extender III containing 15% DMSO, 52.3% (56.3% of the con- trol) for sperm frozen in straws and 48.4% (52.4% of the control) for sperm frozen as pellets. The interaction between extender and cryoprotectant was statistically significant (p<0.05). en_US
dc.format.extent 6 pages en_US
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH en_US
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh en_US
dc.subject cryopreservation, extender, fertilization, Oncorhynchus mykiss, pellet, sperm, straw en_US
dc.subject.lcsh Fish culture--Israel--Periodicals. en_US
dc.subject.lcsh Fish culture--Periodicals. en_US
dc.subject.lcsh Aquaculture--Israel--Periodicals. en_US
dc.subject.lcsh Aquaculture--Periodicals. en_US
dc.title Effect of freezing techniques, extenders and cryoprotectants on the fertilization rate of frozen rainbow trout (Oncorhynchus mykiss) sperm en_US

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