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Effects of molting on the growth of primary cultured muscle cells from and Short Communication
|Title:||Effects of molting on the growth of primary cultured muscle cells from and Short Communication|
|Keywords:||cell culture, Macrobrachium nipponense, molt, Penaeus vannamei, RNA:DNA ratio|
|LC Subject Headings:||Fish culture--Israel--Periodicals.|
|Publisher:||Israeli Journal of Aquaculture - BAMIGDEH|
|Citation:||Wang, W., Wang, A., Nu, D., Liu, Y., & Sun, R. (2005). Effects of molting on the growth of primary cultured muscle cells from and Short Communication. The Israeli Journal of Aquaculture - Bamidgeh, 57(4), 264-270.|
|Series/Report no.:||The Israeli Journal of Aquaculture - Bamidgeh|
|Abstract:||This study examined the effects of molting stage on the growth of primary-cultured cells from the shrimps Macrobrachium nipponense and Penaeus vannamei. Muscle cells in different molting stages were cultured in L-15 medium, supplemented with 1g/l glucose,1g/l MgCl2,1 g/l CaCl2,100 IU/ml penicillin, and 100 μg/ml streptomycin. Results showed that cells cultured in the early postmolt stage grew best. They had an unfolding cell shape, a compact monolayer, a high rate of coverage, and the highest RNA:DNA ratio. Differences among early postmolt, premolt, and intermolt cells were significant (p<0.05).|
|Appears in Collections:||IJA Volume 57, Issue 4, 2005|
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