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Effects of molting on the growth of primary cultured muscle cells from and Short Communication

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Title: Effects of molting on the growth of primary cultured muscle cells from and Short Communication
Authors: Wang, Wei-Na
Wang, An-Li
Nu, Dong-Hong
Liu, Yi-Chen
Sun, Ru-Yong
Keywords: cell culture, Macrobrachium nipponense, molt, Penaeus vannamei, RNA:DNA ratio
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.
Issue Date: 2005
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Wang, W., Wang, A., Nu, D., Liu, Y., & Sun, R. (2005). Effects of molting on the growth of primary cultured muscle cells from and Short Communication. The Israeli Journal of Aquaculture - Bamidgeh, 57(4), 264-270.
Series/Report no.: The Israeli Journal of Aquaculture - Bamidgeh
Abstract: This study examined the effects of molting stage on the growth of primary-cultured cells from the shrimps Macrobrachium nipponense and Penaeus vannamei. Muscle cells in different molting stages were cultured in L-15 medium, supplemented with 1g/l glucose,1g/l MgCl2,1 g/l CaCl2,100 IU/ml penicillin, and 100 μg/ml streptomycin. Results showed that cells cultured in the early postmolt stage grew best. They had an unfolding cell shape, a compact monolayer, a high rate of coverage, and the highest RNA:DNA ratio. Differences among early postmolt, premolt, and intermolt cells were significant (p<0.05).
Pages/Duration: 7 pages
URI/DOI: http://hdl.handle.net/10524/19154
ISSN: 0792-156X
Appears in Collections:IJA Volume 57, Issue 4, 2005



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