Item Description

Show full item record

Title: Genetic basis of sex determination in fishes: Searching for master key regulator genes in the sex determination pathway of tilapias 
Author: Shirak, Andrey; Seroussi, Eyal; Zilberman, Noam; Hulata, Gideon; Ron, Micha; Cnaani, Avner; Kocher, Thomas D.
Date: 2006
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Shirak, A., Seroussi, E., Zilberman, N., Hulata, G., Ron, M., Cnaani, A., & Kocher, T.D. (2006). Genetic basis of sex determination in fishes: Searching for master key regulator genes in the sex determination pathway of tilapias. The Israeli Journal of Aquaculture - Bamidgeh, 58(4), 350-350.
Abstract: Recent results show a striking similarity in the dynamics of expression of gonad differentiation regulators in zebrafish and mammals. Candidates for the role of master key regulators (MKRs) in tilapia were selected, based on three concepts established in the literature for non-mammal species. These three concepts are: (a) MKRs are DM-proteins or their close downstream/ upstream partners in the sex determination pathway, (b) MKRs are genes working just upstream of aromatase in the sex determination pathway, or the aromatase itself, and (c) MKRs are homologs of mammal genes which are close partners of SRY (“missing” in non-mammal verte- brates), mostly belonging to the SOX family. Coding sequences of putative genes were searched in cichlid (TIGR) and general (NCBI) databases, and in the tilapia gonad EST library (RBEST). Primers in two adjacent exons were designed, based on predicted exon-intron boundaries for each of 11 selected genes. Amplified segments of the targeted genes in two purebred tilapia species were sequenced. Seven SSLP and four SNP-based markers were identified in the can- didate genes for MKRs of sex determination and mapped to the tilapia genetic map using geno- type data of 76-90 individuals of the F2 mapping family. The mapping positions of the selected genes relative to previously reported QTL regions for sex determination are discussed.
Series/Report No.: The Israeli Journal of Aquaculture - Bamidgeh
Pages/Duration: 1 pages
ISSN: 0792-156X
URI: http://hdl.handle.net/10524/19194
Keywords: genome mapping, candidate genes, sex determination, tilapia, Oreochromis spp.
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.

Item File(s)

Files Size Format View
58_4_Shirak.pdf 292.4Kb PDF View/Open

This item appears in the following Collection(s)

Search


Advanced Search

Browse

My Account

Statistics

About