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dc.contributor.author Shirak, Andrey en_US
dc.contributor.author Seroussi, Eyal en_US
dc.contributor.author Zilberman, Noam en_US
dc.contributor.author Hulata, Gideon en_US
dc.contributor.author Ron, Micha en_US
dc.contributor.author Cnaani, Avner en_US
dc.contributor.author Kocher, Thomas D. en_US
dc.date.accessioned 2012-01-19T20:18:57Z en_US
dc.date.available 2012-01-19T20:18:57Z en_US
dc.date.issued 2006 en_US
dc.identifier.citation Shirak, A., Seroussi, E., Zilberman, N., Hulata, G., Ron, M., Cnaani, A., & Kocher, T.D. (2006). Genetic basis of sex determination in fishes: Searching for master key regulator genes in the sex determination pathway of tilapias. The Israeli Journal of Aquaculture - Bamidgeh, 58(4), 350-350. en_US
dc.identifier.issn 0792-156X en_US
dc.identifier.uri http://hdl.handle.net/10524/19194 en_US
dc.description.abstract Recent results show a striking similarity in the dynamics of expression of gonad differentiation regulators in zebrafish and mammals. Candidates for the role of master key regulators (MKRs) in tilapia were selected, based on three concepts established in the literature for non-mammal species. These three concepts are: (a) MKRs are DM-proteins or their close downstream/ upstream partners in the sex determination pathway, (b) MKRs are genes working just upstream of aromatase in the sex determination pathway, or the aromatase itself, and (c) MKRs are homologs of mammal genes which are close partners of SRY (“missing” in non-mammal verte- brates), mostly belonging to the SOX family. Coding sequences of putative genes were searched in cichlid (TIGR) and general (NCBI) databases, and in the tilapia gonad EST library (RBEST). Primers in two adjacent exons were designed, based on predicted exon-intron boundaries for each of 11 selected genes. Amplified segments of the targeted genes in two purebred tilapia species were sequenced. Seven SSLP and four SNP-based markers were identified in the can- didate genes for MKRs of sex determination and mapped to the tilapia genetic map using geno- type data of 76-90 individuals of the F2 mapping family. The mapping positions of the selected genes relative to previously reported QTL regions for sex determination are discussed. en_US
dc.format.extent 1 pages en_US
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH en_US
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh en_US
dc.subject genome mapping, candidate genes, sex determination, tilapia, Oreochromis spp. en_US
dc.subject.lcsh Fish culture--Israel--Periodicals. en_US
dc.subject.lcsh Fish culture--Periodicals. en_US
dc.subject.lcsh Aquaculture--Israel--Periodicals. en_US
dc.subject.lcsh Aquaculture--Periodicals. en_US
dc.title Genetic basis of sex determination in fishes: Searching for master key regulator genes in the sex determination pathway of tilapias en_US

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