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dc.contributor.author Kocher, Thomas D. en_US
dc.contributor.author Lee, Bo-Young en_US
dc.contributor.author Cnaani, Avner en_US
dc.date.accessioned 2012-01-19T20:19:10Z en_US
dc.date.available 2012-01-19T20:19:10Z en_US
dc.date.issued 2006 en_US
dc.identifier.citation Kocher, T.D., Lee, B., & Cnaani, A. (2006). Genomic approaches to identifyingsex-determininggenesintilapia. The Israeli Journal of Aquaculture - Bamidgeh, 58(4), 373-373. en_US
dc.identifier.issn 0792-156X en_US
dc.identifier.uri http://hdl.handle.net/10524/19197 en_US
dc.description.abstract Variation in sex determination mechanisms among the different tilapia species has been recog- nized for over 40 years. We have looked for associations between microsatellite DNA markers and sex in families from different species and strains of tilapia. We found that at least two dif- ferent linkage groups are involved in sex determination in this group of fishes. In two species, O. niloticus and T. zillii, we found evidence for male heterogamety with a major sex-determining locus on linkage group 1 (LG1). In two other species, O. aureus (Israeli strain) and O. karongae, we found evidence for female heterogamety with a major locus for sex determination on LG3. In O. aureus (Egyptian strain) and in O. mossambicus, loci associated with sex determination were found on both LG1 and LG3, and a complex mechanism of sex determination was detected. Physical mapping by fluorescence in situ hybridization (FISH) suggests that LG3 corresponds to the largest chromosome pair, and that there is recombination suppression in the sex determina- tion region. The sex-determining region in O. niloticus has been mapped to an 11cM region between markers GM201 and UNH995 on LG1. A BAC contig containing UNH995 was identi- fied and several BACs in the contig were end- or shotgun sequenced. BLAST analysis of these sequences identified Tetraodon chromosome 5 as the homolog of tilapia LG1. Additional SNP and microsatellite markers were developed from published cichlid ESTs and the order of these markers is consistent between tilapia and Tetraodon. We have narrowed the sex-determining region to a 2.6cM interval which corresponds to a 400 kb region of Tetraodon chr5. We are com- pleting genetic and physical maps across this region in order to identify the gene(s) responsible for sex determination in this species. en_US
dc.format.extent 1 pages en_US
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH en_US
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh en_US
dc.subject sex determination, tilapia, linkage map, microsatellite marker en_US
dc.subject.lcsh Fish culture--Israel--Periodicals. en_US
dc.subject.lcsh Fish culture--Periodicals. en_US
dc.subject.lcsh Aquaculture--Israel--Periodicals. en_US
dc.subject.lcsh Aquaculture--Periodicals. en_US
dc.title Genomic approaches to identifyingsex-determininggenesintilapia en_US

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