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dc.contributor.author Xylouri, Eftychia en_US
dc.contributor.author Kotzamanis, Yannis R en_US
dc.contributor.author Athanassopoulou, Fotini en_US
dc.contributor.author Dong, Li en_US
dc.contributor.author Pappas, Loanis S. en_US
dc.contributor.author Argyrokastritis, Alexandros en_US
dc.contributor.author Fragkiadaki. Eirini en_US
dc.date.accessioned 2012-01-19T20:19:53Z en_US
dc.date.available 2012-01-19T20:19:53Z en_US
dc.date.issued 2007 en_US
dc.identifier.citation Xylouri, E., Kotzamanis, Y.R., Athanassopoulou, F., Dong, L., Pappas, L.S., Argyrokastritis, A., & Fragkiadaki.E. (2007). Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities. The Israeli Journal of Aquaculture - Bamidgeh, 59(1), 36-41. en_US
dc.identifier.issn 0792-156X en_US
dc.identifier.uri http://hdl.handle.net/10524/19207 en_US
dc.description.abstract The study demonstrates the presence of a nodavirus that affected sturgeon in fresh water, caus- ing disease with neurological signs. The virus was isolated and inoculated onto SSN-1 (striped snakehead, Channa striatus) cell cultures where cytopathic effects (CPE) of the virus included vacuolation of the cells and degeneration of the monolayer. A 255 bp amplicon from nucleic acid preparations of brain tissue from infected sturgeon was detected by PCR (RT-PCR and nested- PCR) and compared with corresponding amino acid sequences of other infected species. The sequences from the sturgeon were similar to those of sea bass, red spotted grouper, and European eel, supporting the hypothesis that the virus originated in marine fish and was hori- zontally transmitted to freshwater sturgeon. en_US
dc.format.extent 6 pages en_US
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH en_US
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh en_US
dc.subject Acipenser sp., nodavirus, isolation, characterization, sequencing en_US
dc.subject.lcsh Fish culture--Israel--Periodicals. en_US
dc.subject.lcsh Fish culture--Periodicals. en_US
dc.subject.lcsh Aquaculture--Israel--Periodicals. en_US
dc.subject.lcsh Aquaculture--Periodicals. en_US
dc.title Isolation, characterization, and sequencing of nodavirus in sturgeon (Acipenser gueldestaedi L.) reared in freshwater facilities en_US

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