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Item Description Altun, S. en_US Adiloglu, A.K. en_US Kubllay, A. en_US Diler, O. en_US Delibas, N. en_US Sutcu, R. en_US 2012-01-19T20:20:10Z 2012-01-19T20:20:10Z 2007 en_US
dc.identifier.citation Altun, S., Adiloglu, A.K., Kubllay, A., Diler, O., Delibas, N., & Sutcu, R. (2007). Immunogenic and Antigenic Profiles of Nine Lactococcus garvieae Strains from Different Rainbow Trout Farms. The Israeli Journal of Aquaculture - Bamidgeh, 59(2), 111-116. en_US
dc.identifier.issn 0792-156X en_US
dc.description.abstract The aims of this study were to determine differences with respect to immunogenic potency in the antigenic profiles of nine Lactococcus garvieae strains from Turkey, Spain, and England, to develop a bacterin, and to examine the immunological response of rainbow trout to the bacterin. The strains had identical Western blot patterns with 30, 37, 40, 46, 52, and 66 kDA mw protein bands. After culturing the bacteria, proteins were separated by SDS-PAGE and transferred to Immobilon membranes. The membranes were incubated with hyperimmune rabbit sera obtained by immunizing a rabbit against L. garvieae. One group of 50 fish was immunized with formalin- killed bacterin prepared from the most immunogenic strain of L. garvieae. A second group of 50 fish served as an unimmunized control. Four weeks after vaccination, both groups were chal- lenged intraperitoneally with a homologous strain. The protection rate of the bacterin was judged by the relative percent survival (RPS) of the groups. A significantly high level of protection was achieved in the vaccinated group (88.8% RPS). en_US
dc.format.extent 6 pages en_US
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH en_US
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh en_US
dc.subject Oncorhynchus mykiss, Lactococcus garvieae, immunization, Western blot en_US
dc.subject.lcsh Fish culture--Israel--Periodicals. en_US
dc.subject.lcsh Fish culture--Periodicals. en_US
dc.subject.lcsh Aquaculture--Israel--Periodicals. en_US
dc.subject.lcsh Aquaculture--Periodicals. en_US
dc.title Immunogenic and Antigenic Profiles of Nine Lactococcus garvieae Strains from Different Rainbow Trout Farms en_US

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