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dc.contributor.author Mali, P. en_US
dc.contributor.author Maji, S. en_US
dc.contributor.author Joardar, S.N. en_US
dc.date.accessioned 2012-01-19T20:20:28Z en_US
dc.date.available 2012-01-19T20:20:28Z en_US
dc.date.issued 2007 en_US
dc.identifier.citation Mali, P., Maji, S., & Joardar, S.N. (2007). Antigenic Characterization of Outer Membrane Protein of Aeromonas sobria Isolated from Goldfish (Carassius auratus L.). The Israeli Journal of Aquaculture - Bamidgeh, 59(2), 91-98. en_US
dc.identifier.issn 0792-156X en_US
dc.identifier.uri http://hdl.handle.net/10524/19215 en_US
dc.description.abstract Aeromonas sobria, along with A. hydrophila, has frequently been reported as a causative agent of motile aeromonas septicemia (MAS) in fish and other aquatic organisms. Currently, there are no precise tools to diagnose or vaccinate against this disease. The aim of the present study was to fractionate and characterize the outer membrane protein (OMP) antigen of A. sobria by sero- logical and cellular techniques so as to identify immunoreactive molecules that might be useful in preparing immunodiagnostic and/or immunoprophylactic tools against A. sobria infection in goldfish. Eight fractions were isolated from the crude OMP antigen using sephacryl S-200 and DEAE-cellulose chromatography. The highest seroreactivity was observed in the gel-permeated protein G1 which had an optical density (OD) of 0.72 nm, higher even than that of the crude OMP antigen, 0.63 nm. The serodiagnostic potential of G1 was assessed by using dip-stick ELISA. The in vitro goldfish lympho-proliferation ability of the fractionated antigen, G1A3, was higher than of all the other fractionated antigens and the crude OMP. Therefore, fractionated antigen G1 (molecular wt 42-67 kDa) and G1A3 (molecular wt 45-47 kDa) should be further studied in immunodiagnostic and/or immunoprophylactic preparations for A. sobria infection. en_US
dc.format.extent 8 pages en_US
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH en_US
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh en_US
dc.subject Aeromonas sobria, ELISA, fractionation, outer membrane protein (OMP) en_US
dc.subject.lcsh Fish culture--Israel--Periodicals. en_US
dc.subject.lcsh Fish culture--Periodicals. en_US
dc.subject.lcsh Aquaculture--Israel--Periodicals. en_US
dc.subject.lcsh Aquaculture--Periodicals. en_US
dc.title Antigenic Characterization of Outer Membrane Protein of Aeromonas sobria Isolated from Goldfish (Carassius auratus L.) en_US

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