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Isolation of Putative Probionts from the Intestines of Indian Major Carps

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Title: Isolation of Putative Probionts from the Intestines of Indian Major Carps
Authors: Ghosh, Shubhadeep
Sinha, Archana
Sahu, Chittaranjan
Keywords: probiotics, antibacterial activity, Bacillus subtilis, non-pathogen, isolation, adherence, biochemical characterization
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Issue Date: 2007
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Ghosh, S., Sinha, A., & Sahu, C. (2007). Isolation of Putative Probionts from the Intestines of Indian Major Carps. The Israeli Journal of Aquaculture - Bamidgeh, 59(3), 127-132.
Series/Report no.: The Israeli Journal of Aquaculture - Bamidgeh
Abstract: Probiotic strains were isolated from the intestines of 28 Indian major carps (Labeo rohita, Catla catla, Cirrhinus mrigala) cultured in sewage-fed ponds. Intestinal microflora were isolated, count- ed, and identified, and their in vitro antibacterial properties were determined. The double layer method revealed that four strains (SG1, SG2, SG3, SG4) elicited antagonism against the pathogen Pseudomonas fluorescens. Using cross streak and parallel streak methods, SG4 max- imally inhibited the fish pathogens Pseudomonas fluorescens, Aeromonas hydrophila, and Edwardsiella tarda. Therefore, SG4 was chosen for further study as a probiont. SG4 was non- pathogenic to C. mrigala fingerlings, and adhered well to stainless steel, high density polyethyl- ene, and glass plate substrates. Biochemical tests showed that SG4 was endospore-forming, aer- obic, motile, rod-shaped Bacillus subtilis. However, since in vitro experiments can only give an indication of in vivo success, future research should include large-scale in vivo experiments.
Pages/Duration: 6 pages
ISSN: 0792-156X
Appears in Collections:IJA Volume 59, Issue 3, 2007

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