Isolation of Putative Probionts from the Intestines of Indian Major Carps

Date
2007
Authors
Ghosh, Shubhadeep
Sinha, Archana
Sahu, Chittaranjan
Contributor
Advisor
Department
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Annotator
Journal Title
Journal ISSN
Volume Title
Publisher
Israeli Journal of Aquaculture - BAMIGDEH
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
Probiotic strains were isolated from the intestines of 28 Indian major carps (Labeo rohita, Catla catla, Cirrhinus mrigala) cultured in sewage-fed ponds. Intestinal microflora were isolated, count- ed, and identified, and their in vitro antibacterial properties were determined. The double layer method revealed that four strains (SG1, SG2, SG3, SG4) elicited antagonism against the pathogen Pseudomonas fluorescens. Using cross streak and parallel streak methods, SG4 max- imally inhibited the fish pathogens Pseudomonas fluorescens, Aeromonas hydrophila, and Edwardsiella tarda. Therefore, SG4 was chosen for further study as a probiont. SG4 was non- pathogenic to C. mrigala fingerlings, and adhered well to stainless steel, high density polyethyl- ene, and glass plate substrates. Biochemical tests showed that SG4 was endospore-forming, aer- obic, motile, rod-shaped Bacillus subtilis. However, since in vitro experiments can only give an indication of in vivo success, future research should include large-scale in vivo experiments.
Description
Keywords
probiotics, antibacterial activity, Bacillus subtilis, non-pathogen, isolation, adherence, biochemical characterization, Fish culture--Israel--Periodicals., Fish culture--Periodicals., Aquaculture--Israel--Periodicals., Aquaculture--Periodicals.
Citation
Ghosh, S., Sinha, A., & Sahu, C. (2007). Isolation of Putative Probionts from the Intestines of Indian Major Carps. The Israeli Journal of Aquaculture - Bamidgeh, 59(3), 127-132.
Extent
6 pages
Format
Geographic Location
Time Period
Related To
Table of Contents
Rights
Rights Holder
Local Contexts
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.