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Use of PCR-RFLP Analysis of mtDNA Cytochrome-b Gene to Determine Genetic Differences in Capoeta spp.

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Title: Use of PCR-RFLP Analysis of mtDNA Cytochrome-b Gene to Determine Genetic Differences in Capoeta spp.
Authors: Aksakal, Ecrument
Erdogan, Orhan
Keywords: Capoeta spp., Cytochrome-b, MtDNA, PCR, RFLP
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.
Issue Date: 2007
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Aksakai, E., & Erdogan, O. (2007). Use of PCR-RFLP Analysis of mtDNA Cytochrome-b Gene to Determine Genetic Differences in Capoeta spp.. The Israeli Journal of Aquaculture - Bamidgeh, 59(4), 206-211.
Series/Report no.: The Israeli Journal of Aquaculture - Bamidgeh
Abstract: Genetic differences between Capoeta capoeta capoeta, Capoeta capoeta umbla, and Capoeta tinca were determined using PCR-RFLP of mtDNA cytochrome-b (Cyt-b) by amplifying approxi- mately 400-500 bp of this region from each of the three subspecies. The restriction enzymes SpeI and HinfI did not indicate genetic differences but AluI and HpaII did. Thus, PCR-RFLP of the mtDNA was used to distinguish between closely related subspecies without having to ana- lyze the entire DNA sequence of specimens. Use of this technique demonstrated that the Cyt-b regions of the three subspecies have different base sequences. The similarity between C. c. capoeta and C. c. umbla is 71.4% while C. tinca is more distant (50% for C. c. umbla and 33.3% for C. c. capoeta). The Cyt-b similarity is consistent with morphological and taxonomical similar- ities. PCR-RFLP can serve as a tool for genetically identifying subspecies of fish in nature and in aquaculture.
Pages/Duration: 6 pages
URI/DOI: http://hdl.handle.net/10524/19228
ISSN: 0792-156X
Appears in Collections:IJA Volume 59, Issue 4, 2007



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