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Characterization of Vibrio alginolyticus Isolates from Diseased Cultured Gilthead Sea Bream, Sparus aurata
|Title:||Characterization of Vibrio alginolyticus Isolates from Diseased Cultured Gilthead Sea Bream, Sparus aurata|
show 2 moreAlbayrak, G.
|Keywords:||gilthead sea bream, Vibrio alginolyticus, electrophoresis, RAPD|
|LC Subject Headings:||Fish culture--Israel--Periodicals.|
|Publisher:||Israeli Journal of Aquaculture - BAMIGDEH|
|Citation:||Akayli, T., Timur, G., Aydemir, B., Kiziler, A.R., Coskun, O., Albayrak, G., & Arican, E. (2008). Characterization of Vibrio alginolyticus Isolates from Diseased Cultured Gilthead Sea Bream, Sparus aurata. The Israeli Journal of Aquaculture - Bamidgeh, 60(2), 89-94.|
|Series/Report no.:||The Israeli Journal of Aquaculture - Bamidgeh|
|Abstract:||Biochemical, pathogenical, antigenical, and molecular characteristics of Vibrio alginolyticus iso- lates from diseased cultured gilthead sea bream juveniles (4 and 20 g) were determined by lab- oratory challenge experiments. Intraperitonal challenge resulted in development of the disease but immersion did not. Electrophoresis of outer membrane protein (OMP) and lipopolysaccha- rides (LPS) was performed using sodium dodecyl sulphate poly-acrylamide gel electrophoresis (SDS-PAGE). Molecular typing of Vibrio isolates was conducted using random amplification of polymorphic DNA (RAPD). According to SDS-PAGE of surface antigens of the isolates, profiles of both OMP and LPS were heterogeneous. According to RAPD, isolated bacteria varied genet- ically. The findings reveal differences among V. alginolyticus isolates from diseased cultured gilt- head sea bream in Turkey.|
|Appears in Collections:||IJA Volume 60, Issue 2, 2008|
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