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Title: Analysis and Characterization of Expressed Sequence Tag (EST) and Simple Sequence Repeats (SSR) from Red Sea Bream, Chrysophrys major, and Gilthead Sea Bream, Sparus aurata 
Author: Liu, Yun-Guo; Chen, Song-Lin; Liu, Chun-Ying; Li, Fang-Zheng
Date: 2008
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Liu, Y., Chen, S., Liu, C., & Li, F. (2008). Analysis and Characterization of Expressed Sequence Tag (EST) and Simple Sequence Repeats (SSR) from Red Sea Bream, Chrysophrys major, and Gilthead Sea Bream, Sparus aurata. The Israeli Journal of Aquaculture - Bamidgeh, 60(2), 75-81.
Abstract: A total of 101 and 119 simple sequence repeats (SSR) were obtained from expressed sequence tags (EST) of the red sea bream (Chrysophrys major) and gilthead sea bream (Sparus aurata), respectively. Dimeric repeats were the most abundant class of SSR in both red (70.30%) and gilthead (51.26%) sea bream. Trimeric, tetrameric, and pentameric repeats occurred in decreas- ing proportions, i.e., 21.36%, 6.8%, and 0.97% in red sea bream and 45.38%, 3.36%, and 0 in gilthead. There were no hexameric repeats in EST of either species. The frequency of every class of SSR (dimeric, trimeric, tetrameric, and pentameric) decreased with increasing repeat length. In cross-species amplification of 20 existing microsatellite loci from gilthead sea bream in 20 red sea bream specimens, twelve loci showed positive amplification and four loci showed polymorphic amplification with two or three alleles.
Series/Report No.: The Israeli Journal of Aquaculture - Bamidgeh
Pages/Duration: 7 pages
ISSN: 0792-156X
URI: http://hdl.handle.net/10524/19249
Keywords: red sea bream, gilthead sea bream, EST, SSR, microsatellite
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.

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