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dc.contributor.author Liu, Yun-Guo en_US
dc.contributor.author Chen, Song-Lin en_US
dc.contributor.author Liu, Chun-Ying en_US
dc.contributor.author Li, Fang-Zheng en_US
dc.date.accessioned 2012-01-19T20:23:01Z en_US
dc.date.available 2012-01-19T20:23:01Z en_US
dc.date.issued 2008 en_US
dc.identifier.citation Liu, Y., Chen, S., Liu, C., & Li, F. (2008). Analysis and Characterization of Expressed Sequence Tag (EST) and Simple Sequence Repeats (SSR) from Red Sea Bream, Chrysophrys major, and Gilthead Sea Bream, Sparus aurata. The Israeli Journal of Aquaculture - Bamidgeh, 60(2), 75-81. en_US
dc.identifier.issn 0792-156X en_US
dc.identifier.uri http://hdl.handle.net/10524/19249 en_US
dc.description.abstract A total of 101 and 119 simple sequence repeats (SSR) were obtained from expressed sequence tags (EST) of the red sea bream (Chrysophrys major) and gilthead sea bream (Sparus aurata), respectively. Dimeric repeats were the most abundant class of SSR in both red (70.30%) and gilthead (51.26%) sea bream. Trimeric, tetrameric, and pentameric repeats occurred in decreas- ing proportions, i.e., 21.36%, 6.8%, and 0.97% in red sea bream and 45.38%, 3.36%, and 0 in gilthead. There were no hexameric repeats in EST of either species. The frequency of every class of SSR (dimeric, trimeric, tetrameric, and pentameric) decreased with increasing repeat length. In cross-species amplification of 20 existing microsatellite loci from gilthead sea bream in 20 red sea bream specimens, twelve loci showed positive amplification and four loci showed polymorphic amplification with two or three alleles. en_US
dc.format.extent 7 pages en_US
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH en_US
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh en_US
dc.subject red sea bream, gilthead sea bream, EST, SSR, microsatellite en_US
dc.subject.lcsh Fish culture--Israel--Periodicals. en_US
dc.subject.lcsh Fish culture--Periodicals. en_US
dc.subject.lcsh Aquaculture--Israel--Periodicals. en_US
dc.subject.lcsh Aquaculture--Periodicals. en_US
dc.title Analysis and Characterization of Expressed Sequence Tag (EST) and Simple Sequence Repeats (SSR) from Red Sea Bream, Chrysophrys major, and Gilthead Sea Bream, Sparus aurata en_US

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