Activity of the Digestive Protease, Chymotrypsin, in Larvae of the Cultured Sharpsnout Sea Bream (Diplodus puntazzo)

Date
2008
Authors
Aktulun, Sevim
Süzer, Cüneyt
Kamaci, H. Okan
Coban, Deniz
Saka, Sahin
Firat, Kursat
Contributor
Advisor
Department
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Annotator
Journal Title
Journal ISSN
Volume Title
Publisher
Israeli Journal of Aquaculture - BAMIGDEH
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
Specific and total activity of the digestive protease, chymotrypsin, was studied in cultured sharp- snout sea bream larvae (Diplodus puntazzo) for 35 days from hatching and in a variety of pH lev- els. Activity was detected upon hatching (2.8±0.34 mm total length), before the onset of exoge- nous feeding. Specific chymotrypsin activity exponentially increased from mouth opening on day 3 until day 25, then dropped until the end of the experiment. Total chymotrypsin activity sharply increased to day 10, then continued to increase, but slightly, until the end of the experiment. As expected, pH strongly affected both specific and total chymotryptic activity in the digestive tracts of larvae. Both were significantly lower when pH was acidic (1.5, 3.0, 4.0) than when it was alka- line (8.0, 9.0, 10.0) although there were no significant differences within either the acidic or the alkaline range. Our results indicate that chymotrypsin activity is capable of digesting protein before mouth opening and may be a valuable tool for better understanding the nutritional capa- bilities of young larvae.
Description
Keywords
sharpsnout sea bream larvae, Diplodus puntazzo, chymotrypsin, digestive enzyme, pH, Fish culture--Israel--Periodicals., Fish culture--Periodicals., Aquaculture--Israel--Periodicals., Aquaculture--Periodicals.
Citation
Aktulun, S., Suzer, C., Kamaci, H.O., Coban, D., Saka, S., & Firat, K. (2008). Activity of the Digestive Protease, Chymotrypsin, in Larvae of the Cultured Sharpsnout Sea Bream (Diplodus puntazzo). The Israeli Journal of Aquaculture - Bamidgeh, 60(2), 82-88.
Extent
7 pages
Format
Geographic Location
Time Period
Related To
Table of Contents
Rights
Rights Holder
Local Contexts
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.