Item Description

Show full item record

Title: Koi Herpesvirus: Status of Outbreaks, Diagnosis, Surveillance, and Research 
Author: Yuasa, Kei; Sano, Motohiko
Date: 2009
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Yuasa, K., & Sano, M. (2009). Koi Herpesvirus: Status of Outbreaks, Diagnosis, Surveillance, and Research. The Israeli Journal of Aquaculture - Bamidgeh, 61(3), 169-179.
Abstract: Koi herpesvirus (KHV; Cyprinid herpesvirus 3) is a major threat to common carp and koi carp production worldwide. It is listed by the World Organization for Animal Health (OIE), and has been reported in 26 coun- tries. KHV was designated a Specific Disease by Japanese law in 2003. Japan’s National Research Institute of Aquaculture (NRIA) is a reference laboratory for the disease and conducts confirmatory diagnosis. The num- ber of disease occurrences in Japan peaked in 2004, but has been gradu- ally decreasing since then. The disease occurs mostly during spring and autumn when water temperatures are 16-28°C. In general, conventional polymerase chain reaction (PCR) is the most useful method for diagnosing KHV due to its high sensitivity, high specificity, and rapidity. More recently, real time PCR, nested PCR, and loop-mediated isothermal amplification (LAMP) were developed to diagnose the virus. Reverse transcriptase poly- merase chain reaction (RT-PCR) targets mRNA of KHV to detect the repli- cating virus. An enzyme-linked immunosorbent assay (ELISA) detects anti- KHV antibodies in carp serum of surviving infected fish and is expected to be a tool for surveillance of KHV. However, cross reactions with anti- Cyprinid herpesvirus 1 antibodies in the ELISA need to be resolved. A sys- tematic research project on development of diagnostic and prevention tech- nologies for KHV disease was conducted in 2004-2006 by the NRIA in cooperation with Japanese universities and enterprises and SEAFDEC. The fruitful results of the project are included in this review.
Series/Report No.: The Israeli Journal of Aquaculture - Bamidgeh
Pages/Duration: 11 pages
ISSN: 0792-156X
URI: http://hdl.handle.net/10524/19285
Keywords: koi herpesvirus (KHV), diagnosis, prevention, surveillance
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.

Item File(s)

Files Size Format View
61_3_Kei Yuasa.pdf 381.7Kb PDF View/Open

This item appears in the following Collection(s)

Search


Advanced Search

Browse

My Account

Statistics

About