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Title: Herbal Extract Effects on White Spot Syndrome Virus (WSSV) in Shrimp (Penaeus monodon) 
Author: Loan, Ly T.T.; Uyen, Nguyen H.P.; Phuong, Vo H.; Cuong, Doan V.; Anh, Pham V.N.; Hanh, Nguyen N.; Anh, Le T.T.
Date: 2009
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Loan, L.T.T., Uyen, N.H.P., Phuong, V.H., Cuong, D.V., Anh, P.V.N., Hanh, N.N., & Anh, L.T.T. (2009). Herbal Extract Effects on White Spot Syndrome Virus (WSSV) in Shrimp (Penaeus monodon). The Israeli Journal of Aquaculture - Bamidgeh, 61(3), -.
Abstract: Synthetic drugs and chemicals used in aquaculture cause disadvantageous side effects, while medicines made from medicinal herbs are non-toxic, easy to use, and pollution- free. Many medicinal herbs have potent antiviral properties. The extract of Phyllanthus amarus is a lignan composed of the compounds: niranthin, phyllanthin, and hypophyllanthin which have an impact on the white spot syndrome virus (WSSV) in the shrimp, Penaeus monodon. The virucidal activities of the three substances were tested by mixing them with WSSV, followed by injection into healthy shrimp. The quantity of WSSV DNA on the gills of tested shrimp was measured before and seven days after injecting the mixture. The quantity decreased significantly after injection. Anti-virucidal activities were also assessed by observation of the mortality rates of injected shrimp. The lignan compound inactivated the virus when injected in P. monodon at a dose of 100 mg per kilogram body weight. The survival rate of the lignan injected shrimp was 96.67% , compared to the positive control in which it was only 3.33% .
Series/Report No.: The Israeli Journal of Aquaculture - Bamidgeh
Pages/Duration: 1 pages
ISSN: 0792-156X
URI: http://hdl.handle.net/10524/19295
Keywords: herbal extract, WSSV, virucidal activity, Penaeus monodon, Phyllanthus amarus
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.

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