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Identification and Genotyping of Vibrio ordalii: A Comparison of Different Methods

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Title: Identification and Genotyping of Vibrio ordalii: A Comparison of Different Methods
Authors: Akayli, T.
Timur, G.
Albayrak, G.
Aydemir, B.
Keywords: gilthead sea bream, Vibrio ordalii, serology, SDS-PAGE, RAPD
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.
Issue Date: 2010
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Akayli, T., Timur, G., Albayrak, G., & Aydemir, B. (2010). Identification and Genotyping of Vibrio ordalii: A Comparison of Different Methods. The Israeli Journal of Aquaculture - Bamidgeh, 62(1), 9-18.
Series/Report no.: The Israeli Journal of Aquaculture - Bamidgeh
Abstract: The phenotypical, serological, and molecular identification of Vibrio ordalii isolates recovered from diseased gilthead sea bream (Sparus aurata) in the Mediterranean area were investigated. Most isolates had similar phenotypic characteristics, but some showed small differentiation in biochemical analysis. Both slide agglutination and enzyme linked immunosorbent assay (ELISA) showed that the isolates were antigenically homogeneous and no different serotypes were detected. SDS-PAGE analysis showed that the structure of lipopolysaccharide (LPS) was heterogeneous, that outer membrane proteins (OMP) display homogeneous grouping protein banding patterns, and that the LPS profile of the isolates had different banding patterns. However, random amplified polymorphic DNA (RAPD) analysis revealed genetic variation (up to 90.4%) among isolates of the same species.
Pages/Duration: 10 pages
URI/DOI: http://hdl.handle.net/10524/19305
ISSN: 0792-156X
Appears in Collections:IJA Volume 62, Issue 1, 2010



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