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Title: Induction of All-triploid Japanese Flounder (Paralichthys olivaceus) by Cold Shock 
Author: Xu, Tian-jun; Chen, Song-lin
Date: 2010
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Xu, T., & Chen, S. (2010). Induction of All-triploid Japanese Flounder (Paralichthys olivaceus) by Cold Shock. The Israeli Journal of Aquaculture - Bamidgeh, 62(1), 43-49.
Abstract: Cold shock is a useful method of inducing triploidy in some fish species. In this paper, triploidy in Japanese flounder (Paralichthys olivaceus) was induced by applying cold shocks of 3°C, three or four minutes after fertilization, for 15, 25, 35, 45, 55, or 65 min. Ploidy of fry was analyzed using a ploidy analyzer. Survival was lower in treatments shocked at four minutes than in treatments shocked at three minutes after fertilization, and decreased as the duration of the cold shock increased. The abnormality rate increased with the shock duration. The best results were achieved in the treatment shocked three minutes after fertilization for 25 min, resulting in 100% triploidy with the highest survival rate (93.27%) and one of the lowest abnormality rates (15.87%). This study shows that cold shock is a highly effective method for inducing triploidy in the Japanese flounder.
Series/Report No.: The Israeli Journal of Aquaculture - Bamidgeh
Pages/Duration: 7 pages
ISSN: 0792-156X
URI: http://hdl.handle.net/10524/19309
Keywords: Japanese flounder (Paralichthys olivaceus), triploidy induction, cold shock
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.

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