Effects of Dietary Manganese Supplementation on Antioxidant Enzyme Activity in the Shrimp (Neocaridina heteropoda)

Abstract

The effects of the manganese (Mn) supplemented diets on the level of superoxide anions (O-2) in the hemolymph, and the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) in the muscle of Neocaridina heteropoda were investigated. Manganese sulfate (MnSO4) was added to a basal diet at 0, 20, 40, 60, 80, or 100 μg/g. Diets were fed to triplicate groups of shrimp (0.30±0.11 g, 1.4±0.1 cm) in a recirculating freshwater rearing system for 30 days. The Mn concentration in the rearing water, monitored during feeding, was 1.00±0.02 μg/l. O-2 was lower and antioxidant enzyme activity was higher (p<0.05) in shrimps fed Mn-supplemented diets than in shrimps fed the control. Antioxidant enzyme activity reached a maximum when the Mn concentration was 60 μg/g diet.