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Identification  and  Antimicrobial  Activity  of  Combined  Extract  from  Azadirachta  indica  and  Ocimum  sanctum

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Title: Identification  and  Antimicrobial  Activity  of  Combined  Extract  from  Azadirachta  indica  and  Ocimum  sanctum
Authors: Harikrishnan, Ramasamy
Kim, Man-Chul
Kim, Ju-Sang
Balasundaram, Chellam
Jawahar, Sundaram
show 1 moreHeo, Moon-Soo
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Keywords: Azadirachta indica, Ocimum sanctum, antibacterial activity, bacteria
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Issue Date: 2010
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Harikrishnan, R., Kim, M., Kim, J., Balasundaram, C., Jawahar, S., & Heo, M. (2010). Identification  and  Antimicrobial  Activity  of  Combined  Extract  from  Azadirachta  indica  and  Ocimum  sanctum . The Israeli Journal of Aquaculture - Bamidgeh, 62(2), 85-95.
Series/Report no.: The Israeli Journal of Aquaculture - Bamidgeh
Abstract: The  chemical  constituents  of  decoction  (individual)  and  concoction  (mixed)   of   ethanolic   leaf   extracts   from   Azadirachta   indica   (neem)   and   Ocimum  
sanctum   (tulsi)   were   analyzed   by   gas   chromatography-­mass   spectro-­ photometery  (GC-­MS).  Decoctions  of  A.  indica  and  O.  sanctum  had  24  and   33   constituents,   respectively.   Mixed   together,   26   compounds   were   identified.   Four   major   (high   percentage)   compounds   were   identified   in   A.   indica:   n-­hexadecanoic   acid   (14.34%),   phytol   (19.96%),   9,12,15-­octa-­ decatrienoic   acid,   (Z,Z,Z)-­   (18.57%),   and   vitamin   E   (11.37%).   Three   major  compounds  were  identified  in  O.  sanctum:  phenol,2-­methoxy-­3-­(2-­ propenyl)   (15.32%),   9,12,15-­octadecatrienoic   acid,(Z,Z,Z)-­   (16.94%),   and  9,12,15-­octadecatrienoic  acid,  methyl  ester,(Z,Z,Z)-­  (22.05%).  Three   major   compounds   were   identified   in   the   mixed   extract:   n-­hexadecanoic   acid  (16.58%),  phenol,2-­methoxy-­3-­(2-­propenyl)  (20.62%),  and  9,12,15-­ octadecatrienoic   acid,(Z,Z,Z)   (25.98%).   Four   of   the   compounds   in   the   mixed   extract   were   new:   eudesma-­4(14),11-­diene   (0.18%),   6-­ azabicyclo[3.2.1]octane   (0.51%),   cyclohexane,1-­ethyl-­1-­methyl-­2,4-­ bis(1-­methylenyl)-­,[1S-­␣␣␣␣␣␣␣␣␣␣␣-­␣-­Elemen   (0.77%),   and   globulol   (1.45%).   The   mixed   extract   had   a   high   level   of   antimicrobial   activity   against   fish   pathogens   as   indicated   by   zone   of   inhibition,   minimum   inhibitory  concentration,  and  minimum  bactericidal  concentration.
Pages/Duration: 11 pages
ISSN: 0792-156X
Appears in Collections:IJA Volume 62, Issue 2, 2010

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