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|Title:||Identification and Antimicrobial Activity of Combined Extract from Azadirachta indica and Ocimum sanctum|
|Keywords:||Azadirachta indica, Ocimum sanctum, antibacterial activity, bacteria|
|LC Subject Headings:||Fish culture--Israel--Periodicals.|
|Publisher:||Israeli Journal of Aquaculture - BAMIGDEH|
|Citation:||Harikrishnan, R., Kim, M., Kim, J., Balasundaram, C., Jawahar, S., & Heo, M. (2010). Identification and Antimicrobial Activity of Combined Extract from Azadirachta indica and Ocimum sanctum . The Israeli Journal of Aquaculture - Bamidgeh, 62(2), 85-95.|
|Series/Report no.:||The Israeli Journal of Aquaculture - Bamidgeh|
|Abstract:||The chemical constituents of decoction (individual) and concoction (mixed) of ethanolic leaf extracts from Azadirachta indica (neem) and Ocimum |
sanctum (tulsi) were analyzed by gas chromatography-mass spectro- photometery (GC-MS). Decoctions of A. indica and O. sanctum had 24 and 33 constituents, respectively. Mixed together, 26 compounds were identified. Four major (high percentage) compounds were identified in A. indica: n-hexadecanoic acid (14.34%), phytol (19.96%), 9,12,15-octa- decatrienoic acid, (Z,Z,Z)- (18.57%), and vitamin E (11.37%). Three major compounds were identified in O. sanctum: phenol,2-methoxy-3-(2- propenyl) (15.32%), 9,12,15-octadecatrienoic acid,(Z,Z,Z)- (16.94%), and 9,12,15-octadecatrienoic acid, methyl ester,(Z,Z,Z)- (22.05%). Three major compounds were identified in the mixed extract: n-hexadecanoic acid (16.58%), phenol,2-methoxy-3-(2-propenyl) (20.62%), and 9,12,15- octadecatrienoic acid,(Z,Z,Z) (25.98%). Four of the compounds in the mixed extract were new: eudesma-4(14),11-diene (0.18%), 6- azabicyclo[3.2.1]octane (0.51%), cyclohexane,1-ethyl-1-methyl-2,4- bis(1-methylenyl)-,[1S-␣␣␣␣␣␣␣␣␣␣␣-␣-Elemen (0.77%), and globulol (1.45%). The mixed extract had a high level of antimicrobial activity against fish pathogens as indicated by zone of inhibition, minimum inhibitory concentration, and minimum bactericidal concentration.
|Appears in Collections:||IJA Volume 62, Issue 2, 2010|
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