Effect of Lipopolysaccharide (LPS) and Outer Membrane Protein (OMP) Vaccines on Protection of Grass Carp (Ctenopharyngodon idella) against Aeromonas hydrophila
Effect of Lipopolysaccharide (LPS) and Outer Membrane Protein (OMP) Vaccines on Protection of Grass Carp (Ctenopharyngodon idella) against Aeromonas hydrophila
Date
2011
Authors
Sun, Jinhui
Wang, Qingkui
Qiao, Zhiyi
Bai, Dong-qing
Sun, Jingfeng
Qiao, Xiuting
Contributor
Advisor
Department
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Annotator
Journal Title
Journal ISSN
Volume Title
Publisher
Israeli Journal of Aquaculture - BAMIGDEH
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
The gram-negative bacterium, Aeromonas hydrophila, causes high mortality and economic losses to the aquaculture industry. We investigated whether lipopolysaccharide (LPS) or outer membrane proteins (OMP) from A. hydrophila can enhance specific and/or non-specific immunity in grass carp (Ctenopharyngodon idella). Fish were injected intraperitoneally with LPS, OMP, or formalin-killed cells (FKC) from A. hydrophila. The control group was injected with phosphate buffered saline (PBS). All three antigens elicited strong immune responses. Respiratory burst and phagocytic activities in head kidney leukocytes and serum lysozyme activity peaked on day 21 after vaccination. Heavy chain gene transcription of immunoglobulin M and Z in the head kidney in vaccinated fish peaked on day 28. Relative percent survival was 83.3%, 72.2%, and 55.6% in the LPS, OMP, and FKC groups, respectively, but only 10% in control fish. Results suggest that LPS and OMP isolated from A. hydrophila can enhance specific immunity, non-specific immunity, and protection against A. hydrophila in fish. Thus, LPS and OMP could be important antigens for development of vaccines to control diseases caused by A. hydrophila in grass carp and other aquatic animals.
Description
Keywords
Fish culture--Israel--Periodicals.,
Fish culture--Periodicals.,
Aquaculture--Israel--Periodicals.,
Aquaculture--Periodicals.
Citation
Jinhui Sun, Qingkui Wang, Zhiyi Qiao, Dongqing Bai, Jingfeng Sun, Xiuting Qiao. (2011). Effect of Lipopolysaccharide (LPS) and Outer Membrane Protein (OMP) Vaccines on Protection of Grass Carp (Ctenopharyngodon idella) against Aeromonas hydrophila. The Israeli Journal of Aquaculture - Bamidgeh, 63, 8 pp.
Extent
8 pages
Format
Geographic Location
Time Period
Related To
Table of Contents
Rights
Rights Holder
Local Contexts
Collections
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.