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dc.contributor.author Sun, Jinhui en_US
dc.contributor.author Wang, Qingkui en_US
dc.contributor.author Qiao, Zhiyi en_US
dc.contributor.author Bai, Dongqing en_US
dc.contributor.author Sun, Jingfeng en_US
dc.contributor.author Qiao, Xiuting en_US
dc.date.accessioned 2012-03-02T22:08:16Z en_US
dc.date.available 2012-03-02T22:08:16Z en_US
dc.date.issued 2011 en_US
dc.identifier.citation Jinhui Sun, Qingkui Wang, Zhiyi Qiao, Dongqing Bai, Jingfeng Sun, Xiuting Qiao. (2011). Effect of Lipopolysaccharide (LPS) and Outer Membrane Protein (OMP) Vaccines on Protection of Grass Carp (Ctenopharyngodon idella) against Aeromonas hydrophila. The Israeli Journal of Aquaculture - Bamidgeh, 63, 8 pp. en_US
dc.identifier.issn 0792-156X en_US
dc.identifier.other IIC:63.2011.655
dc.identifier.uri http://hdl.handle.net/10524/22913 en_US
dc.description.abstract The gram-negative bacterium, Aeromonas hydrophila, causes high mortality and economic losses to the aquaculture industry. We investigated whether lipopolysaccharide (LPS) or outer membrane proteins (OMP) from A. hydrophila can enhance specific and/or non-specific immunity in grass carp (Ctenopharyngodon idella). Fish were injected intraperitoneally with LPS, OMP, or formalin-killed cells (FKC) from A. hydrophila. The control group was injected with phosphate buffered saline (PBS). All three antigens elicited strong immune responses. Respiratory burst and phagocytic activities in head kidney leukocytes and serum lysozyme activity peaked on day 21 after vaccination. Heavy chain gene transcription of immunoglobulin M and Z in the head kidney in vaccinated fish peaked on day 28. Relative percent survival was 83.3%, 72.2%, and 55.6% in the LPS, OMP, and FKC groups, respectively, but only 10% in control fish. Results suggest that LPS and OMP isolated from A. hydrophila can enhance specific immunity, non-specific immunity, and protection against A. hydrophila in fish. Thus, LPS and OMP could be important antigens for development of vaccines to control diseases caused by A. hydrophila in grass carp and other aquatic animals. en_US
dc.format.extent 8 pages en_US
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH en_US
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh en_US
dc.subject.lcsh Fish culture--Israel--Periodicals. en_US
dc.subject.lcsh Fish culture--Periodicals. en_US
dc.subject.lcsh Aquaculture--Israel--Periodicals. en_US
dc.subject.lcsh Aquaculture--Periodicals. en_US
dc.title Effect of Lipopolysaccharide (LPS) and Outer Membrane Protein (OMP) Vaccines on Protection of Grass Carp (Ctenopharyngodon idella) against Aeromonas hydrophila en_US

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