Please use this identifier to cite or link to this item:
Visualization of Sparus macrocephalus Infection by GFP-Labeled Edwardsiella tarda
|Title:||Visualization of Sparus macrocephalus Infection by GFP-Labeled Edwardsiella tarda|
show 1 moreXie, Qilang
|LC Subject Headings:||Fish culture--Israel--Periodicals.|
|Publisher:||Israeli Journal of Aquaculture - BAMIGDEH|
|Citation:||Maocang Yan,Xuepeng Wang, Weili Hu, Shaobo Chen, Saile Zhang, Qilang Xie. (2012). Visualization of Sparus macrocephalus Infection by GFP-Labeled Edwardsiella tarda. The Israeli Journal of Aquaculture - Bamidgeh, 64, 7 pp.|
|Series/Report no.:||The Israeli Journal of Aquaculture - Bamidgeh|
|Abstract:||Edwardsiella tarda is a fish pathogen that causes systemic infections in many marine and freshwater fish. The invasion pathway of E. tarda was studied in vivo in Sparus macrocephalus using a virulent strain of E. tarda, transformed with a plasmid encoding green fluorescent protein and sharing similar virulence characteristics as the parent strain. Fish were divided into six groups: (A) bacteria bath-challenged, unwounded fish, (B) bacteria bath-challenged fish, skin mucus layer partially removed by paper towel, (C) bacteria bath-challenged fish, skin artificially wounded by scalpel, (D) bacteria intramuscularly-challenged fish, (E) normal fish, and (F) phosphate-buffered saline intramuscularly-treated fish. The number of bacteria from blood, gills, kidney, muscle, spleen, liver, and intestine were determined 2, 4, 6, 12, 24, and 48 h post-challenge. High numbers of bacteria were observed in the gills and intestine, followed by the liver, kidney, spleen, and muscle of infected fish. The number of bacteria was significantly higher in groups B and C than in group A. The skin, intestine, and gills were likely the main routes of entry for E. tarda.|
|Appears in Collections:||Volume 64, 2012|
Please contact email@example.com if you need this content in an alternative format.
Items in eVols are protected by copyright, with all rights reserved, unless otherwise indicated.