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Visualization of Sparus macrocephalus Infection by GFP-Labeled Edwardsiella tarda

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Title: Visualization of Sparus macrocephalus Infection by GFP-Labeled Edwardsiella tarda
Authors: Yan, Maocang
Wang, Xuepeng
Hu, Weili
Chen, Shaobo
Zhang, Saile
show 1 moreXie, Qilang
show less
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Issue Date: 2012
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Maocang Yan,Xuepeng Wang, Weili Hu, Shaobo Chen, Saile Zhang, Qilang Xie. (2012). Visualization of Sparus macrocephalus Infection by GFP-Labeled Edwardsiella tarda. The Israeli Journal of Aquaculture - Bamidgeh, 64, 7 pp.
Series/Report no.: The Israeli Journal of Aquaculture - Bamidgeh
Abstract: Edwardsiella tarda is a fish pathogen that causes systemic infections in many marine and freshwater fish. The invasion pathway of E. tarda was studied in vivo in Sparus macrocephalus using a virulent strain of E. tarda, transformed with a plasmid encoding green fluorescent protein and sharing similar virulence characteristics as the parent strain. Fish were divided into six groups: (A) bacteria bath-challenged, unwounded fish, (B) bacteria bath-challenged fish, skin mucus layer partially removed by paper towel, (C) bacteria bath-challenged fish, skin artificially wounded by scalpel, (D) bacteria intramuscularly-challenged fish, (E) normal fish, and (F) phosphate-buffered saline intramuscularly-treated fish. The number of bacteria from blood, gills, kidney, muscle, spleen, liver, and intestine were determined 2, 4, 6, 12, 24, and 48 h post-challenge. High numbers of bacteria were observed in the gills and intestine, followed by the liver, kidney, spleen, and muscle of infected fish. The number of bacteria was significantly higher in groups B and C than in group A. The skin, intestine, and gills were likely the main routes of entry for E. tarda.
Pages/Duration: 7 pages
ISSN: 0792-156X
Appears in Collections:Volume 64, 2012

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