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dc.contributor.author Yan, Maocang en_US
dc.contributor.author Wang, Xuepeng en_US
dc.contributor.author Hu, Weili en_US
dc.contributor.author Chen, Shaobo en_US
dc.contributor.author Zhang, Saile en_US
dc.contributor.author Xie, Qilang en_US
dc.date.accessioned 2012-03-02T22:08:49Z en_US
dc.date.available 2012-03-02T22:08:49Z en_US
dc.date.issued 2012 en_US
dc.identifier.citation Maocang Yan,Xuepeng Wang, Weili Hu, Shaobo Chen, Saile Zhang, Qilang Xie. (2012). Visualization of Sparus macrocephalus Infection by GFP-Labeled Edwardsiella tarda. The Israeli Journal of Aquaculture - Bamidgeh, 64, 7 pp. en_US
dc.identifier.issn 0792-156X en_US
dc.identifier.other IJA_64.2012.693
dc.identifier.uri http://hdl.handle.net/10524/22920 en_US
dc.description.abstract Edwardsiella tarda is a fish pathogen that causes systemic infections in many marine and freshwater fish. The invasion pathway of E. tarda was studied in vivo in Sparus macrocephalus using a virulent strain of E. tarda, transformed with a plasmid encoding green fluorescent protein and sharing similar virulence characteristics as the parent strain. Fish were divided into six groups: (A) bacteria bath-challenged, unwounded fish, (B) bacteria bath-challenged fish, skin mucus layer partially removed by paper towel, (C) bacteria bath-challenged fish, skin artificially wounded by scalpel, (D) bacteria intramuscularly-challenged fish, (E) normal fish, and (F) phosphate-buffered saline intramuscularly-treated fish. The number of bacteria from blood, gills, kidney, muscle, spleen, liver, and intestine were determined 2, 4, 6, 12, 24, and 48 h post-challenge. High numbers of bacteria were observed in the gills and intestine, followed by the liver, kidney, spleen, and muscle of infected fish. The number of bacteria was significantly higher in groups B and C than in group A. The skin, intestine, and gills were likely the main routes of entry for E. tarda. en_US
dc.format.extent 7 pages en_US
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH en_US
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh en_US
dc.subject.lcsh Fish culture--Israel--Periodicals. en_US
dc.subject.lcsh Fish culture--Periodicals. en_US
dc.subject.lcsh Aquaculture--Israel--Periodicals. en_US
dc.subject.lcsh Aquaculture--Periodicals. en_US
dc.title Visualization of Sparus macrocephalus Infection by GFP-Labeled Edwardsiella tarda en_US

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