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A Direct Spectrophotometric Assay for Evaluating Nitrate-Nitrogen in Intensive Aquaculture Systems
|Title:||A Direct Spectrophotometric Assay for Evaluating Nitrate-Nitrogen in Intensive Aquaculture Systems|
Rocchina Caputo, Anna
|LC Subject Headings:||Fish culture--Israel--Periodicals.|
|Publisher:||Israeli Journal of Aquaculture - BAMIGDEH|
|Citation:||Stefano Cecchini, Anna Rocchina Caputo. (2012). A Direct Spectrophotometric Assay for Evaluating Nitrate-Nitrogen in Intensive Aquaculture Systems. The Israeli Journal of Aquaculture - Bamidgeh, 64, 6 pp.|
|Series/Report no.:||The Israeli Journal of Aquaculture - Bamidgeh|
|Abstract:||This paper describes a simple spectrophotometric procedure for determining NO3--N in water samples. In the proposed method, NO2--N of a water sample is first removed from the water with sulfamic acid. Then, NO3--N is reduced to NO2--N using vanadium (III) chloride and a detection reagent is added. When rapid collection of data is necessary, this method can be adapted to a microtechnique using a 96-well microplate, resulting in a detection limit of 5 NO3--N mg/l. Using a larger volume spectrophotometer cell (5 cm) lowers the detection limit to 0.5 NO3--N mg/l. The proposed method is applicable for routine analysis of water in aquaculture systems and wastewaters in which medium-high NO3--N concentrations are expected. Benefits of the method are reduction of the laborious preparation and avoidance of contact with the harmful substances typical of the cadmium reduction method.|
|Appears in Collections:||Volume 64, 2012|
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