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Title: A Direct Spectrophotometric Assay for Evaluating Nitrate-Nitrogen in Intensive Aquaculture Systems
Authors: Cecchini, Stefano
Rocchina Caputo, Anna
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Issue Date: 2012
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Stefano Cecchini, Anna Rocchina Caputo. (2012). A Direct Spectrophotometric Assay for Evaluating Nitrate-Nitrogen in Intensive Aquaculture Systems. The Israeli Journal of Aquaculture - Bamidgeh, 64, 6 pp.
Series/Report no.: The Israeli Journal of Aquaculture - Bamidgeh
Abstract: This paper describes a simple spectrophotometric procedure for determining NO3--N in water samples. In the proposed method, NO2--N of a water sample is first removed from the water with sulfamic acid. Then, NO3--N is reduced to NO2--N using vanadium (III) chloride and a detection reagent is added. When rapid collection of data is necessary, this method can be adapted to a microtechnique using a 96-well microplate, resulting in a detection limit of 5 NO3--N mg/l. Using a larger volume spectrophotometer cell (5 cm) lowers the detection limit to 0.5 NO3--N mg/l. The proposed method is applicable for routine analysis of water in aquaculture systems and wastewaters in which medium-high NO3--N concentrations are expected. Benefits of the method are reduction of the laborious preparation and avoidance of contact with the harmful substances typical of the cadmium reduction method.
Pages/Duration: 6 pages
ISSN: 0792-156X
Appears in Collections:Volume 64, 2012

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