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|Title:||A Loop-Mediated Isothermal Amplification Method Targeting the fbsB Gene for Rapid Detection of Streptococcus agalactiae, the Causative Agent of Streptococcosis in Farmed Fish|
show 2 moreJian, J.C.
|LC Subject Headings:||Fish culture--Israel--Periodicals.|
|Publisher:||Israeli Journal of Aquaculture - BAMIGDEH|
|Citation:||Wang B., Cai S.H., Lu Y.S., Zhang X.L., Huang Y.C., Jian J.C., Wu Z.H. (2012). A Loop-Mediated Isothermal Amplification Method Targeting the fbsB Gene for Rapid Detection of Streptococcus agalactiae, the Causative Agent of Streptococcosis in Farmed Fish. The Israeli Journal of Aquaculture - Bamidgeh, 64, 7 pp.|
|Series/Report no.:||The Israeli Journal of Aquaculture - Bamidgeh|
|Abstract:||The purpose of this study was to develop a loop-mediated isothermal amplification (LAMP) method for rapid, sensitive, and simple detection of Streptococcus agalactiae in farmed fish. A set of four primers, two outer and two inner, was designed from the fibrinogen-binding (fbsB) proteins gene sequence of S. agalactiae, and conditions for LAMP were optimized as incubation of all reagents for 60 min at 64°C. LAMP products were judged by agar gel electrophoresis as well as the naked eye after development by Synergy Brands Green I (SYBR Green I). Sensitivity of the assay was 2.8 × 103 CFU/ml (2.8 CFU per reaction) and no positive amplification was detected for other related bacteria. This LAMP method can be used to detect S. agalactiae infection in the laboratory and the field.|
|Appears in Collections:||Volume 64, 2012|
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