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dc.contributor.author Wang, B. en_US
dc.contributor.author Cai, S.H. en_US
dc.contributor.author Lu, Y.S. en_US
dc.contributor.author Zhang, X.L. en_US
dc.contributor.author Huang, Y.C. en_US
dc.contributor.author Jian, J.C. en_US
dc.contributor.author Wu, Z.H. en_US
dc.date.accessioned 2012-03-02T22:09:07Z en_US
dc.date.available 2012-03-02T22:09:07Z en_US
dc.date.issued 2012 en_US
dc.identifier.citation Wang B., Cai S.H., Lu Y.S., Zhang X.L., Huang Y.C., Jian J.C., Wu Z.H. (2012). A Loop-Mediated Isothermal Amplification Method Targeting the fbsB Gene for Rapid Detection of Streptococcus agalactiae, the Causative Agent of Streptococcosis in Farmed Fish. The Israeli Journal of Aquaculture - Bamidgeh, 64, 7 pp. en_US
dc.identifier.issn 0792-156X en_US
dc.identifier.other IJA_64.2012.677
dc.identifier.uri http://hdl.handle.net/10524/22924 en_US
dc.description.abstract The purpose of this study was to develop a loop-mediated isothermal amplification (LAMP) method for rapid, sensitive, and simple detection of Streptococcus agalactiae in farmed fish. A set of four primers, two outer and two inner, was designed from the fibrinogen-binding (fbsB) proteins gene sequence of S. agalactiae, and conditions for LAMP were optimized as incubation of all reagents for 60 min at 64°C. LAMP products were judged by agar gel electrophoresis as well as the naked eye after development by Synergy Brands Green I (SYBR Green I). Sensitivity of the assay was 2.8 × 103 CFU/ml (2.8 CFU per reaction) and no positive amplification was detected for other related bacteria. This LAMP method can be used to detect S. agalactiae infection in the laboratory and the field. en_US
dc.format.extent 7 pages en_US
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH en_US
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh en_US
dc.subject.lcsh Fish culture--Israel--Periodicals. en_US
dc.subject.lcsh Fish culture--Periodicals. en_US
dc.subject.lcsh Aquaculture--Israel--Periodicals. en_US
dc.subject.lcsh Aquaculture--Periodicals. en_US
dc.title A Loop-Mediated Isothermal Amplification Method Targeting the fbsB Gene for Rapid Detection of Streptococcus agalactiae, the Causative Agent of Streptococcosis in Farmed Fish en_US

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