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dc.contributor.author Kushwaha, J.P. en_US
dc.contributor.author Sridhar, N. en_US
dc.contributor.author Kumar, U.V. en_US
dc.contributor.author Prasanth, K.P.H. en_US
dc.contributor.author Raghunath, M.R. en_US
dc.contributor.author Eknath, A.E. en_US
dc.date.accessioned 2012-03-02T22:09:21Z en_US
dc.date.available 2012-03-02T22:09:21Z en_US
dc.date.issued 2012 en_US
dc.identifier.citation Kushwaha J.P., Sridhar N., Kumar U.V., Prasanth K.P.H., Raghunath M.R., Eknath A.E. (2012). Partial Purification and Characterization of Amylases from the Digestive Tract of the Indian Medium Carp Labeo fimbriatus (Bloch, 1797). The Israeli Journal of Aquaculture - Bamidgeh, 64, 8 pp. en_US
dc.identifier.issn 0792-156X en_US
dc.identifier.other IJA_64.2012.686
dc.identifier.uri http://hdl.handle.net/10524/22927 en_US
dc.description.abstract Partial purification of amylases from the digestive tract of the Indian medium carp Labeo fimbriatus through acetone fractionation and Sephadex G-75 gel filtration resulted in 5-fold purification with 29% recovery. Characterization of amylase activity revealed two pH optima at 4.5 and 6.5-7.0. Activity was stable over a wide pH range of 4.0-10.0. Optimum incubation temperature was 25°C. The enzyme lost 80% activity at 50°C within 30 min and was inhibited by 1 mM p-chloromercuri benzoate, ethylene diamine tetra-acetate, and phenyl methyl sulphonyl fluoride. The heavy metal ions Hg++, Cd++, Cu++, Zn++, Fe++, Pb++, Bi++, and Ag+ strongly inhibited enzyme activity whereas Ca++ activated it. Native polyacrylamide gel electrophoresis of the purified amylase fractions revealed four bands, with corresponding molecular weights of 72, 68, 66, and 65 kDa. Amylase activity from L. fimbriatus exhibited linear hydrolysis of starch up to 7% concentration. en_US
dc.format.extent 8 pages en_US
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH en_US
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh en_US
dc.subject.lcsh Fish culture--Israel--Periodicals. en_US
dc.subject.lcsh Fish culture--Periodicals. en_US
dc.subject.lcsh Aquaculture--Israel--Periodicals. en_US
dc.subject.lcsh Aquaculture--Periodicals. en_US
dc.title Partial Purification and Characterization of Amylases from the Digestive Tract of the Indian Medium Carp Labeo fimbriatus (Bloch, 1797) en_US

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