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dc.contributor.author Marhual, N.P. en_US
dc.contributor.author Das, B.K. en_US
dc.contributor.author Pradhan, J. en_US
dc.contributor.author Swain, P. en_US
dc.contributor.author Mishra, B.K. en_US
dc.contributor.author Eknath, A.E. en_US
dc.date.accessioned 2012-03-02T22:09:30Z en_US
dc.date.available 2012-03-02T22:09:30Z en_US
dc.date.issued 2012 en_US
dc.identifier.citation Marhual N.P., Das B.K., Pradhan J., Swain P., Mishra B.K., Eknath A.E. (2012). RAPD-PCR and Outer Membrane Protein Characterization of Vibrio alginolyticus and Vibrio parahaemolyticus Isolated from Diseased shrimp. The Israeli Journal of Aquaculture - Bamidgeh, 64, 8 pp. en_US
dc.identifier.issn 0792-156X en_US
dc.identifier.other IJA_64.2012.683
dc.identifier.uri http://hdl.handle.net/10524/22929 en_US
dc.description.abstract Vibrio alginolyticus and V. parahaemolyticus were characterized by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and profiling of outer membrane proteins (OMP) to the genetic level to enable easier identification of these microorganisms from diseased shrimp (Penaeus monodon) and adopt control measures. Of 20 tested Operon primers (OPC), only OPC5 and OPC18 were used for PCR amlification of 70 isolates representing three strains of each species. OPC5 amplified 1-8 bands with sizes ranging 0.42-2.95 Kb in V. alginolyticus, 5-10 fragments with sizes ranging 0.449-3.55 Kb in V. parahaemolyticus, and one unique common band of 1.34 Kb in both species. When OPC18 was used, one dark band (2.4 Kb) was common to all V. alginolyticus strains and another dark band (1.77 Kb) was common to two strains and the V. alginolyticus ATCC reference strain. One protein band (37.63 kDa) was common to both V. alginolyticus and V. parahaemolyticus and could be used as a diagnostic OMP marker for both species. The simpler and more rapid dot-ELISA and indirect ELISA methods confirmed identification of these pathogenic bacteria. en_US
dc.format.extent 8 pages en_US
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH en_US
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh en_US
dc.subject.lcsh Fish culture--Israel--Periodicals. en_US
dc.subject.lcsh Fish culture--Periodicals. en_US
dc.subject.lcsh Aquaculture--Israel--Periodicals. en_US
dc.subject.lcsh Aquaculture--Periodicals. en_US
dc.title RAPD-PCR and Outer Membrane Protein Characterization of Vibrio alginolyticus and Vibrio parahaemolyticus Isolated from Diseased shrimp en_US

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