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Title: An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas 
Author: Zhang, Guofan; Wang, Xiaotong; Song, Xiaorui; Li, Li
Date: 2012
Abstract: We earlier developed a method to extract DNA from in vivo-sampled mantle tissue of the bivalve Pacific oyster (Crassostrea gigas) for molecular marker-assisted selection (MAS) in breeding. However, mortality was too high when using this method. In the current study, a more efficient and safer method of extracting DNA from the oyster shell was developed, based on the improved phenol-chloroform DNA extraction method. Both nuclear DNA and mitochondrial DNA were successfully extracted from the oyster shell, and mortality was 0%. These results illustrate the safety of extracting DNA from the bivalve shell. In addition, because DNA is a cellular component, the results provide molecular evidence that cells are likely involved in shell formation.
Series/Report No.: The Israeli Journal of Aquaculture - Bamidgeh
Pages/Duration: 4 pages
ISSN: 0792-156X
Identifier: IJA_64.2012.800
Keywords: mollusc, bivalve, Pacific oyster, shell DNA, marker-assisted selection (MAS)
LC Subject Headings: Fish culture--Israel.
Fish culture.

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