Please use this identifier to cite or link to this item: http://hdl.handle.net/10524/31832

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Title: An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas
Authors: Zhang, Guofan
Wang, Xiaotong
Song, Xiaorui
Li, Li
Keywords: mollusc
bivalve
Pacific oyster
shell DNA
marker-assisted selection (MAS)
LC Subject Headings: Fish culture--Israel.
Fish culture.
Issue Date: 2012
Series/Report no.: The Israeli Journal of Aquaculture - Bamidgeh
Abstract: We earlier developed a method to extract DNA from in vivo-sampled mantle tissue of the bivalve Pacific oyster (Crassostrea gigas) for molecular marker-assisted selection (MAS) in breeding. However, mortality was too high when using this method. In the current study, a more efficient and safer method of extracting DNA from the oyster shell was developed, based on the improved phenol-chloroform DNA extraction method. Both nuclear DNA and mitochondrial DNA were successfully extracted from the oyster shell, and mortality was 0%. These results illustrate the safety of extracting DNA from the bivalve shell. In addition, because DNA is a cellular component, the results provide molecular evidence that cells are likely involved in shell formation.
Pages/Duration: 4 pages
URI: http://hdl.handle.net/10524/31832
ISSN: 0792-156X
Appears in Collections:Volume 64, 2012



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