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Item Description Zhang, Guofan en_US Wang, Xiaotong en_US Song, Xiaorui en_US Li, Li en_US 2012-10-15T23:33:08Z 2012-10-15T23:33:08Z 2012 en_US
dc.identifier.issn 0792-156X en_US
dc.identifier.other IJA_64.2012.800 en_US
dc.description.abstract We earlier developed a method to extract DNA from in vivo-sampled mantle tissue of the bivalve Pacific oyster (Crassostrea gigas) for molecular marker-assisted selection (MAS) in breeding. However, mortality was too high when using this method. In the current study, a more efficient and safer method of extracting DNA from the oyster shell was developed, based on the improved phenol-chloroform DNA extraction method. Both nuclear DNA and mitochondrial DNA were successfully extracted from the oyster shell, and mortality was 0%. These results illustrate the safety of extracting DNA from the bivalve shell. In addition, because DNA is a cellular component, the results provide molecular evidence that cells are likely involved in shell formation. en_US
dc.format.extent 4 pages en_US
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh en_US
dc.subject mollusc en_US
dc.subject bivalve en_US
dc.subject Pacific oyster en_US
dc.subject shell DNA en_US
dc.subject marker-assisted selection (MAS) en_US
dc.subject.lcsh Fish culture--Israel. en_US
dc.subject.lcsh Fish culture. en_US
dc.title An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas en_US

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