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Effect of Glycerol on Fertility of Cryopreserved Grass Carp (Ctenopharyngodon idella) Sperm

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Title: Effect of Glycerol on Fertility of Cryopreserved Grass Carp (Ctenopharyngodon idella) Sperm
Authors: Bozkurt, Yusuf
Yavaş, İlker
Öğretmen, Fatih
Sivaslıgil, Buğra
Karaca, Fikret
Keywords: grass carp
Ctenopharyngodon idella
cryopreservation
glycerol
extender
show 2 moresemen
fertilization

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LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.
Issue Date: 2011
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Yusuf Bozkurt, İlker Yavaş, Fatih Öğretmen, Buğra Sivaslıgil, Fikret Karaca (2011). Effect of Glycerol on Fertility of Cryopreserved Grass Carp (Ctenopharyngodon idella) Sperm. The Israeli Journal of Aquaculture - Bamidgeh, 63, 6 pp.
Series/Report no.: The Israeli Journal of Aquaculture - Bamidgeh
Abstract: This research investigated the effect of adding glycerol to different ionic extenders on motility and fertilizing ability of frozen-thawed sperm of grass carp (Ctenopharyngodon idella) under hatchery conditions. Semen was collected by abdominal stripping from adult males and diluted to a ratio of 1:3 in Kurokura I or Kurokura II extender containing 10%, 15%, or 20% glycerol. Diluted semen was packaged in 0.5-ml straws and left to equilibrate for 30 min at 4°C. Following equilibration, the straws were exposed to liquid nitrogen vapor for 10 min and plunged into liquid nitrogen (-196°C). Straws were thawed in a water bath at 30°C for 10 s to determine the post-thaw motility and movement duration. The spermatozoa:egg ratio in fertilization experiments was 1 x 105:1. The highest mean fertilization rate (95.63±0.52; p>0.05) was obtained with semen frozen in Kurokura I extender containing 10% glycerol. Results indicate that grass carp sperm can be successfully cryopreserved with ionic extenders containing glycerol at 10-20%.
Pages/Duration: 6 pages
URI/DOI: http://hdl.handle.net/10524/36291
ISSN: 0792-156X
Appears in Collections:IJA Volume 63, 2011



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