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Partial Purification and Characterization of the Inhibitory Substance of Bacillus subtilis against Common Bacterial Fish Pathogens

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Title: Partial Purification and Characterization of the Inhibitory Substance of Bacillus subtilis against Common Bacterial Fish Pathogens
Authors: Nayak, S.K.
Mukherjee, S.C.
Keywords: antimicrobial activity
Bacillus subtilis
extra cellular protein (ECP) fraction
fish pathogens
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.
Issue Date: 2011
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: S.K. Nayak and S.C. Mukherjee (2011). Partial Purification and Characterization of the Inhibitory Substance of Bacillus subtilis against Common Bacterial Fish Pathogens. The Israeli Journal of Aquaculture - Bamidgeh, 63, 5 pp.
Series/Report no.: The Israeli Journal of Aquaculture - Bamidgeh
Abstract: The present investigation was carried out to study the active component of Bacillus subtilis, a gastrointestinal bacterium of mrigal (Cirrhinus mrigala) that shows inhibitory activity against a wide range of fish pathogens. The bacterium was found to inhibit all the fish pathogens and an extra cellular protein fraction (ECP) of 5-10 kD was found to be the active component responsible for inhibitory activity. The component was heat stable (60°C for 1 h) and a change in pH had no effect on the antibacterial activity. The protein fraction was sensitive to trypsin, chymotrypsin, lysozyme, and proteinase K at 2.5 mg/ml and resistant at 0.1 and 0.5 mg/ml. The present investigation confirmed that the active component of the B. subtilis responsible for antibacterial activities against common fish pathogens is a low molecular weight protein fraction (5-10 kD). A protein or peptide with such a low molecular weight has the potential to serve as an alternative health management strategy for combating disease in aquaculture.
Pages/Duration: 5 pages
URI/DOI: http://hdl.handle.net/10524/36292
ISSN: 0792-156X
Appears in Collections:IJA Volume 63, 2011



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