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Partial Purification and Characterization of the Inhibitory Substance of Bacillus subtilis against Common Bacterial Fish Pathogens
|Title:||Partial Purification and Characterization of the Inhibitory Substance of Bacillus subtilis against Common Bacterial Fish Pathogens|
extra cellular protein (ECP) fraction
|LC Subject Headings:||Fish culture--Israel--Periodicals.|
|Publisher:||Israeli Journal of Aquaculture - BAMIGDEH|
|Citation:||S.K. Nayak and S.C. Mukherjee (2011). Partial Purification and Characterization of the Inhibitory Substance of Bacillus subtilis against Common Bacterial Fish Pathogens. The Israeli Journal of Aquaculture - Bamidgeh, 63, 5 pp.|
|Series/Report no.:||The Israeli Journal of Aquaculture - Bamidgeh|
|Abstract:||The present investigation was carried out to study the active component of Bacillus subtilis, a gastrointestinal bacterium of mrigal (Cirrhinus mrigala) that shows inhibitory activity against a wide range of fish pathogens. The bacterium was found to inhibit all the fish pathogens and an extra cellular protein fraction (ECP) of 5-10 kD was found to be the active component responsible for inhibitory activity. The component was heat stable (60°C for 1 h) and a change in pH had no effect on the antibacterial activity. The protein fraction was sensitive to trypsin, chymotrypsin, lysozyme, and proteinase K at 2.5 mg/ml and resistant at 0.1 and 0.5 mg/ml. The present investigation confirmed that the active component of the B. subtilis responsible for antibacterial activities against common fish pathogens is a low molecular weight protein fraction (5-10 kD). A protein or peptide with such a low molecular weight has the potential to serve as an alternative health management strategy for combating disease in aquaculture.|
|Appears in Collections:||IJA Volume 63, 2011|
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