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Evaluation of Berberine’s Algicidal Effects on Toxic Microcystis aeruginosa Growth using a Double Fluorescein Staining Method

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Title: Evaluation of Berberine’s Algicidal Effects on Toxic Microcystis aeruginosa Growth using a Double Fluorescein Staining Method
Authors: Zhang, Shulin
Zhang, Mingming
Dai, Wei
Bi, Xiangdong
Yang, Pan
show 1 moreZhang, Dajuan
show less
Keywords: Microcystis aeruginosa
fluorescein diacetate
propidium iodide
berberine
LC Subject Headings: Fish culture--Israel.
Fish culture.
Issue Date: 2014
Abstract: An accurate and convenient method for determining Microcystis cell viability is necessary to control blooms in small aquaculture water bodies. The fluorescein diacetate-propidium iodide (FDA-PI) staining method was developed to determine the viability of toxic Microcystis aeruginosa (FACHB905). Live M. aeruginosa 905 cells, stained with FDA, emitted bright green fluorescence after excitation at 470 nm. Dead cells, stained with PI, produced bright red fluorescence after excitation at 540 nm. Berberine inhibited M. aeruginosas 905 growth and the inhibition rate determined by FDA-PI fluorescein staining and counting on a dark field was much higher than when determined by counting on a bright field because some berberine-killed cells were misidentified as live cells. Thus, less berberine is needed to control Microcystis bloom when the accurate and reliable FDA-PI fluorescein staining method is used to judge the viability of the algae cells.
Pages/Duration: 5 pages
URI/DOI: http://hdl.handle.net/10524/49113
ISSN: 0792-156X
Appears in Collections:Volume 66, 2014



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