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Isolation and Infectious Temperature Optimization of Genetically Similar VHSV Isolates in Farmed Olive Flounder, Paralichthys olivaceus

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Title: Isolation and Infectious Temperature Optimization of Genetically Similar VHSV Isolates in Farmed Olive Flounder, Paralichthys olivaceus
Authors: Jang, Yeoung-Hwan
Dharaneedharan, Subramanian
Kang, Bong-Jo
Heo, Moon-Soo
Keywords: viral hemorrhagic septicemia virus
VHS
N-gene
infection temperature
flounder fish
LC Subject Headings: Fish culture--Israel.
Fish culture.
Issue Date: 2014
Abstract: Viral hemorrhagic septicemia virus (VHSV) was isolated from farmed olive flounder (Paralichthys olivaceus). The viral N-gene was amplified by reverse transcriptase PCR, cloned, and sequenced for phylogenetic analysis to identify the genotype (I-IV). Virus isolates were cultured on Epithelioma papulosum cyprini cell line and, after completion of the cytopathic effects, the supernatant was collected and used to challenge virus-free flounder. The infected founder were reared in 16°C, 21°C, or 25°C, and compared to an unchallenged control. Virus titration was measured in the head kidneys, spleens, livers, brains, muscles, and gills of challenged fish using real-time quantification of the VHSV G-gene. Phylogenetic analysis confirmed that the isolates were VHSV Genogroup IV. The VHSV-challenged fish in the 16°C group showed 100% mortality with significantly increased expression of viral G-gene mRNA in the spleen, compared to fish reared in other temperatures and the control fish, suggesting that fish reared in 16°C are more susceptible to VHSV infection.
Pages/Duration: 7 pages
URI/DOI: http://hdl.handle.net/10524/49118
ISSN: 0792-156X
Appears in Collections:Volume 66, 2014



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