Triploidy Induction by Heat Shock in Mandarin Fish Siniperca chuatsi

Abstract

Mandarin fish, Siniperca chuatsi, is an economically important fish due to its large size, fast growth, and delicious flesh. It is widely cultured in China. In this paper, triploidy in mandarin fish S. chuatsi was induced by heat shock. The most effective triploidy induction was achieved at 41°C, 8 min after fertilization for 2 min resulting in 40% triploid fish. There were no significant differences in the survival rates among the three treatment groups. Ploidy of fish was determined with a flow cytometer and chromosome counting. In conclusion, this paper presents optimal conditions for triploidy induction in mandarin fish with heat shock. The results will contribute to enhancement of its production in culture.