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New rRNA primers for the detection of Vibrio anguillarum

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Title: New rRNA primers for the detection of Vibrio anguillarum
Authors: Avsever, Meriç Lütfi
Tunalıgil, Serra
Didinen, Behire Işıl
Metin, Seçil
Keywords: Vibrio anguillarum
LC Subject Headings: Fish culture--Israel.
Fish culture.
Issue Date: 2015
Abstract: In this work, 51 V. anguillarum isolates (42/51 of O1 serotype, 9/51 of O2) obtained from cultivated marine fish by Avsever and Ün (2015) were used. These isolates were confirmed with universal primers specific to amiB genes. They were also detected successfully at a rate of 100% with a new primer couple designed according to 16S ribosomal RNA sequence. The new primers were found to have a high sensitivity and specificity but were liable to give a cross-reaction with Vibrio ordalii.
Pages/Duration: 6 pages
ISSN: 0792-156X
Appears in Collections:Volume 67, 2015

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