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The Effect of Muscle-Regulatory Factor Genes and Satellite Cell Response to Recombinant Hsp70 Protein on Megalobrama amblycephala Skeletal Muscle
|Title:||The Effect of Muscle-Regulatory Factor Genes and Satellite Cell Response to Recombinant Hsp70 Protein on Megalobrama amblycephala Skeletal Muscle|
|LC Subject Headings:||Fish culture--Israel.|
|Abstract:||Hsp70 (70-kDa heat shock protein) plays a key regulatory role in muscle development. In order to evaluate whether recombinant Hsp70 protein (rMaHsp70) of M. amblycephala could affect muscle growth by inducing the expression of Myogenin (MyoG), Myogenic Differentiation Antigen (MyoD), Myogenic factor 5 (Myf5), myogenic regulatory factor 4 (MRF4) and myostatin (MSTN) in muscle, Hsp70 was injected intraperitoneally (IP) at two concentrations: 1.0 mg/mL and 3.4 mg/mL. The mRNA and protein levels of the transcription factor Pax-7 and the protein Hsp70 initially increased, then as concentration levels of injected recombinant M. amblycephala Hsp70 protein rMaHsp70 further increased, the protein Hsp70 decreased. In order to investigate the relationship between muscle hyperplasia, hypertrophy and satellite cell growth following rMaHsp70 administration, the number of satellite cells were analyzed and revealed that the numbers of satellite cells in low concentration group (1 mg/mL) was predominantly higher than in the other concentration groups in both red and white muscle. These results suggest that low concentrations of rMaHsp70 could decrease muscle-related gene expression and increase the number of satellite cells. Our research will be helpful in adapting Hsp70 as a feed additive to enhance M. amblycephala growth.|
|Appears in Collections:||Volume 68, 2016|
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