Please use this identifier to cite or link to this item: http://hdl.handle.net/10524/57165

Identification and mRNA Expression of Antioxidant Enzyme Genes in the Mud Crab (Scylla paramamosain) in Response to Acute Ammonia and Nitrite Exposure

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Title:Identification and mRNA Expression of Antioxidant Enzyme Genes in the Mud Crab (Scylla paramamosain) in Response to Acute Ammonia and Nitrite Exposure
Authors:Chenga, C-H
Maa, H.-L.
Fenga, J.
Sua, Y-L.
Denga, Y-Q.
show 2 morePenga, J-H.
Guo, Z-X
show less
Keywords:Scylla paramamosain
antioxidant enzyme genes
thioredoxin reductase
ambient stressors
LC Subject Headings:Fish culture--Israel.
Fish culture
Date Issued:2018
Abstract:Thioredoxin reductase (TrxR) is a conserved protein that is involved in protecting organisms against various oxidative stresses. In this study, a thioredoxin reductase gene was cloned from the mud crab Scylla paramamosain (SpTrxR). The full-length cDNA of SpTrxR is comprised of 2724 bp with a 1791 bp open reading frame that encodes a putative protein of 596 amino acids. The deduced amino acid sequence of SpTrxR contains the typical TrxR domain. Quantitative real-time PCR analysis revealed that the SpTrxR mRNA was distributed abundantly in mud crabs, while strong expression was observed mainly in the gills. The expression of antioxidant enzyme genes (SpTrxR, SpTrx, SpSOD, and SpCAT) was measured using quantitative real-time PCR after acute ammonia and nitrite exposure. The results show that antioxidant enzyme genes (SpTrxR, SpTrx, SpSOD, and SpCAT) were modulated by acute ammonia and nitrite exposure. These results suggest that antioxidant enzyme genes play an important role in protecting organisms against oxidative stress
Pages/Duration:13 pages
URI/DOI:http://hdl.handle.net/10524/57165
ISSN:0792-156X
Appears in Collections: Volume 70, 2018


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