Please use this identifier to cite or link to this item: http://hdl.handle.net/10524/57166

Two Rapid Methods to Identify Three Species of Pathogenic Vibrio in Penaeus vannamei

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dc.contributor.author Luo, K.
dc.contributor.author Yuan, H.
dc.contributor.author Zhang, W.
dc.contributor.author Xu, Q.
dc.date.accessioned 2018-05-11T19:58:09Z
dc.date.available 2018-05-11T19:58:09Z
dc.date.issued 2018
dc.identifier.issn 0792-156X
dc.identifier.uri http://hdl.handle.net/10524/57166
dc.description.abstract Vibrio species frequently infect Penaeus vannamei causing great economic losses to the Whiteleg shrimp industry. Rapid detection of pathogenic Vibrio infection would improve the fight against these diseases. In this study, single and multiple polymerase chain reaction (PCR) methods were developed to detect three species of pathogenic Vibrio: Vibrio fluvialis, Vibrio anguillarum, and Vibrio alginolyticus. Specific primers were designed for the toxR gene of V. fluvialis, the flaA gene of V. anguillarum, and the pyrH gene of V. alginolyticus. The bacteria were used as templates to establish a 25 μL reaction system for PCR amplification. The results showed that single and specific PCR amplification products of expected sizes were obtained (228bp, 1665bp, and 383bp, respectively). The lowest concentration detected for the three Vibrio species were 5.21×102 , 2.70×104 , and 2.48×102 colony forming units (cfu)/mL, respectively. We also developed a multiplex PCR method to identify the three Vibrio species accurately, and with improved identification efficiency. In addition, quantitative real-time PCR (qPCR) was used to identify the minimum detectable DNA concentration for the three Vibrio species (1.0×10-6 nmol/L for V. fluvialis, 1.0×10-7 nmol/L for V. anguillarum, and 1.0×10- 8 nmol/L for V. alginolyticus). Technical requirements for ordinary PCR are low, therefore PCR is a feasible technique to detect and diagnose Penaeus vannamei bacterial disease.
dc.format.extent 7 pages
dc.subject Penaeus vannamei
dc.subject Vibrio fluvialis
dc.subject Vibrio anguillarum
dc.subject Vibrio alginolyticus
dc.subject multiplex PCR
dc.subject qPCR
dc.subject.lcsh Fish culture--Israel.
dc.subject.lcsh Fish culture
dc.title Two Rapid Methods to Identify Three Species of Pathogenic Vibrio in Penaeus vannamei
dc.type Article
dc.type.dcmi Text
Appears in Collections: Volume 70, 2018


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