Please use this identifier to cite or link to this item: http://hdl.handle.net/10524/57168

Effects of Salinity Fluctuation on Gene Expression Profiles of Female Litopenaeus vannamei Broodstocks

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dc.contributor.author Zhang, M.
dc.contributor.author Huang, X-K
dc.contributor.author Hu, L-H.
dc.contributor.author Ji, D.W.
dc.contributor.author Tang, M
dc.contributor.author Li, F.
dc.contributor.author Luo, K.
dc.contributor.author Zhang, J.M.
dc.contributor.author Chai, X.L.
dc.contributor.author Yan, M.C.
dc.date.accessioned 2018-05-11T19:58:26Z
dc.date.available 2018-05-11T19:58:26Z
dc.date.issued 2018
dc.identifier.issn 0792-156X
dc.identifier.uri http://hdl.handle.net/10524/57168
dc.description.abstract In this study, the effects of salinity fluctuation on gene expression patterns in ovary and hepatopancreas of Litopenaeus vannamei were analyzed. The expression level of vitellogenin in the ovaries were significantly, markedly, and slightly, inhibited respectively in the hepatopancreas after brooders were transferred from 30‰ to 20‰ or 40‰ salinity for 4 days. The expression levels of the development of germ cell related genes Dmc1, proliferating cell nuclear antigen (PCNA) and cyclophilin A were significantly reduced at 20‰ or 40‰ salinity. The transcript level of shrimp ovarian peritrophin (SOP) was significantly reduced at 20‰ salinity, and the expression level of ovary specific gene mucin-5 AC was significantly higher at 40‰ salinity. Four stress related genes, cytoplasmic carbonic anhydrase (LvCAc), Na+/K+-ATPase alpha subunit (LvNK), V-H+-ATPase subunit A (LvVH), and heat shock protein 90 (LvHSP90), were significantly up-regulated at 40‰ salinity in the ovaries. In the hepatopancreas, the transcript level of fatty acid synthase (LvFAS) was significantly inhibited under 40‰ salinity. The mRNA levels of stress related genes LvNK, LvVH, and LvHSP90, were significantly lower at 40‰ salinity. Results indicated that changes in salinity caused transcription inhibition of germ cell and ovarian development related genes, and the expression patterns of stress related genes in the ovaries and hepatopancreas were quite different.
dc.format.extent 9 pages
dc.subject Litopenaeus vannamei
dc.subject salinity
dc.subject ovary
dc.subject hepatopancreas
dc.subject germ cell
dc.subject Vitellogenin
dc.subject.lcsh Fish culture--Israel.
dc.subject.lcsh Fish culture
dc.title Effects of Salinity Fluctuation on Gene Expression Profiles of Female Litopenaeus vannamei Broodstocks
dc.type Article
dc.type.dcmi Text
Appears in Collections: Volume 70, 2018


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