Pathogenicity and Immune Response of Starry Flounder, Platichthys stellatus, Infected with Vibrio anguillarum

Abstract

Vibrio anguillarum is the aetiological agent of vibriosis, a disease affecting many marine fish species. The occurrence of vibriosis in starry flounder, Platichthys stellatus, grown in an aquaculture farm has demonstrated the urgent need for information on pathogenic infection and immune response for efficient disease management. This is the first study to report Vibrio anguillarum isolation and infection in starry flounder. We evaluated immune responses, serum biochemical parameters, and cumulative mortality of the fish by experimentally challenging healthy fish. The expression levels of five immune genes (TNF, TNFR, IL-6, MHCII, and CXC) were measured by real-time quantitative PCR. The transcriptional levels of the genes encoding tumor necrosis factor (TNF), TNF receptor (TNFR), interleukin-6 (IL-6), the major histocompatibility complex (MHC-II), and a chemokine (CXC) in the head-kidney of V. anguillarum infected fish were significantly upregulated compared with control fish and biochemical indices including the alanine aminotransferase, total serum protein, and glucose levels of infected fish differed significantly from those of control. Additionally, Starry flounder infected with V. anguillarum at 1.67 × 106 and 1.67 × 108CFU/mL showed 53%, and 100% mortality, respectively. This study furthers our understanding of the immune and serum biochemical alterations, and mortality induced by bacterial infections, depending on pathogen concentration. This may advance strategies for control of V. anguillarum in cultured starry flounder