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Parentage Assignment in Striped Catfish (Pangasianodon hypophthalmus) Based on Microsatellite Marke

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Title:Parentage Assignment in Striped Catfish (Pangasianodon hypophthalmus) Based on Microsatellite Marke
Authors:Nguyen M.T
Le Nguyen T.P
Nguyen T.L
Duong A.L
Keywords:parentage assignment
Pangasianodon hypophthalmus
multiplex PCR
Date Issued:2019
Publisher:The Israeli Journal of Aquaculture - Bamidgeh
Abstract:In this study, five microsatellite markers from two multiplex PCR sets were used to determine the pedigree of five striped catfish (Pangasianodon hypophthalmus) families that came from a breeding program in Vietnam. The number of alleles per locus and polymorphic information content (PIC) were 4 to 7 and 0.551-0.803, respectively. In the parentage analysis based on five microsatellites, 62.7% of offspring could be allocated unambiguously to their parental pairs using the perfect mismatch setting, and assignment rate improved to 89.3% after a single mismatch was allowed. Parentage analysis when only four microsatellites were used (a locus with a high frequency of null alleles was excluded) increased the assignment rate to 68% for a perfect mismatch and to 90% for assignment with a single mismatch. Results here confirm that a DNA markerbased approach to parentage assignment in P. hypophthalmus will be useful in genetic improvement programs for this important aquaculture species in Vietnam. .
Pages/Duration:9 pages
Appears in Collections: Volume 71, 2019

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