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Title: In Vitro Melatonin Rhythm Reveals A Clocked Pineal In The European Sea Bass, Dicentrarchus Labrax 
Author: Ron, Benny
Date: 2004
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Ron, B. (2004). In Vitro Melatonin Rhythm Reveals A Clocked Pineal In The European Sea Bass, Dicentrarchus Labrax. The Israeli Journal of Aquaculture - Bamidgeh, 56(4), 281-285.
Abstract: The melatonin-generating system of the European sea bass, Dicentrarchus labrax, was studied in vitro using a perfusion tissue culture methodology. The examined properties included photic entrainment in the pineal gland, and circadian rhythmicity in alternating dark and light, in contin- uous darkness, and in continuous light. Pineal glands were removed from adult fish and placed in a perfusion tissue culture at a constant temperature of 24°C. Melatonin discharge into the cul- ture medium was measured using radioimmunoassay. Melatonin concentrations during a pho- toperiod of 12h light:12h dark were low during the light periods and high during the dark. The melatonin discharge was inhibited by continuous light but strong and rhythmic in continuous dark with a circadian period (Tau) of about 24 h (n = 10). The results demonstrate that the European sea bass pineal gland is photosensitive, with ability to be entrained, and contains an internal cir- cadian oscillator that regulates melatonin production.
Series/Report No.: The Israeli Journal of Aquaculture - Bamidgeh
Pages/Duration: 5 pages
ISSN: 0792-156X
Keywords: circadian oscillator; Dicentrarchus labrax; melatonin; pineal gland
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.

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